Research on the Relationship between Spinal Ways and the Effect of Point Zusanli （ST 36）

目的：横断研究相应脊髓背根通路的完整性对针刺足三里穴后胃平滑肌电活动的影响，探讨中枢性调控对针刺足三里穴效应的联系途径。方法：选择SD健康成年大鼠30只，用冰乙酸法复制幽门管部溃疡模型，随机分为模型对照组、背根L4-S1切断组、T10-T11横断组，每组10只。用直径0．2mm铂金圆环电极植入溃疡部浆膜下，记录针刺前后胃平滑肌电活动的变化，刺激参数：2—100Hz，疏密方波，刺激电流峰值1．5mA，连续刺激20min。结果：模型对照组针刺前为（5．73±0．51）cpm，（2．28±0．57）mV：针刺后，（5．34±0．34）cpm，（1．06±0．13）mV。背根（L4-S1）组切断前，（5．65±0．26）cpm，（1．66±0．15）mV：切断后，（5．40±0．23）cpm，（1．17±0．23）mV，切断加针刺后，（5．26±0．18）cpm，（2．45±0．23）mV。脊髓（T10-T11）组横断前，（5．69±0．36）cpm，（1．91±0．25）mV：横断后，（4．98±0．53）cpm，（1．05±0．18）mV。横断加针刺后，（4．60±0．44）cpm，（2．52±0．38）mV。结论：针刺足三里具有抑制胃溃疡模型大鼠胃平滑肌电活动的作用；切断相应背根后，针刺足三里有增强胃平滑肌电幅值的作用，且横断脊髓作用更强，表明脊髓通路的完整性直接影响针刺效应，针刺的双向调节作用与脊髓以上高位中枢的调控机制密切相关。

Research on the relationship between spinal ways and the effect of point Zusanli (ST 36)

Objective  The integrity of the corresponding dorsal root is important in the research of the effects of acupuncture Zusanli (ST 36) on gastric smooth muscle. Investigate the communicate channels of central control on the effect of acupuncture Zusanli (ST 36). Method  Selected 30 healthy adult SD rats and using veniua-glacial acid to made gastric mucosa ulcer model. Divided them into three groups, model group, cutting L₄-S₁ group and transecting T₁₀-T₁₁ group after the models have been made successfully, each group 10 rats. Implanted diameter in 0.2 mm Platinum Circle electrode into subserous of the ulcer place and recorded the myoelectricity activity of gastric smooth muscle before and after acupuncture Zusanli (ST 36). Acupuncture parameters, 2–100 Hz, lasting 20 min. ResultsModel group before acupuncture, (5.73 ± 0.51) cycle per minitue (cpm), (2.28 ± 0.57) mV; model group after acupuncture, (5.34 ± 0.34) cpm, (1.06 ± 0.13). Group that before cutting dorsal L₄-S₁, (5.65 ± 0.26) cpm, (1.66 ± 0.15) mV; group that after cutting dorsal L₄-S₁, (5.40 ± 0.23) cpm, (1.17 ± 0.23), group that after cutting dorsal L₄-S₁ and acupuncture, (5.26 ± 0.18) cpm, (2.45 ± 0.23). Group that before transecting dorsal T₁₀-T₁₁, (5.69 ± 0.36) cpm, (1.91 ± 0.25) mV, group that after transecting dorsal T₁₀-T₁₁, (4.98 ± 0.53) cpm, (1.05 ± 0.18) mV, group that after transecting dorsal T₁₀-T₁₁ and acupuncture, (4.60 ± 0.44) cpm, (2.52 ± 0.38) mV   Conclusion  Acupunture Zusanli (ST 36) can inhibit the myoelectricity activity of gastric smooth muscle, cutting L₄-S₁ can strengthen the myoelectricity activity, but the effect of transect T₁₀–T₁₁ is better. So it can be said that the integrity of Dorsal root is important to the effect of acupuncture. The two-way regulation of Acupuncture has a close relationship with the higher centre above spinal cord.