| 骨髓间充质干细胞体外诱导分化为神经样细胞的机制 |
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| 引用本文： | 褚倩,王亚平,傅新巧,张苏明.骨髓间充质干细胞体外诱导分化为神经样细胞的机制[J].中国康复理论与实践,2004,10(1):13-14. |
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| 作者姓名： | 褚倩 王亚平 傅新巧 张苏明 |
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| 作者单位： | 430030,湖北武汉市,华中科技大学同济医学院附属同济医院神经科 |
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| 基金项目： | 国家自然科学基金资助项目 (No .30 0 70 82 5)，中国博士后科学基金资助项目 (No .2 0 0 2 0 32 2 1 5) |
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| 摘 要： | 目的探索骨髓间充质干细胞 (MSCs)在体外诱导分化为神经样细胞的机制。方法分离培养大鼠MSCs ,用二甲基亚砜 (DSMO)和丁羟茴醚 (BHA)诱导分化 ,检测诱导分化前、预诱导 2 4h、诱导分化后 6h、2 4h和 48h神经细胞和神经干细胞的特异性标记蛋白的表达。结果诱导分化后 ,大部分MSCs变成双极、多极和锥形 ,并相互交织成网络结构 ,巢蛋白 (Nestin)在诱导分化前不表达 ,在诱导分化后 6h达到最高 ,2 4h和 48h逐渐降低。神经元特异性核蛋白 (NeuN )在诱导分化前不表达 ,在诱导分化后 6h出现表达 ,2 4h和 48h表达增强。结论MSCs经体外诱导先分化为神经干细胞 ,然后分化为神经元样细胞。
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| 关 键 词： | 骨髓间充质干细胞 分化 神经元样细胞 神经干细胞 |
| 文章编号： | 1006-9771(2004)01-0013-02 |
| 修稿时间： | 2003年12月21 |
Mechanism of differentiation of mesenchymal stem cells into neuron-like cells in vitro |
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| CHU Qian,WANG Ya-ping,FU Xin-qiao,et al..Mechanism of differentiation of mesenchymal stem cells into neuron-like cells in vitro[J].Chinese Journal of Rehabilitation Theory and Practice,2004,10(1):13-14. |
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| Authors: | CHU Qian WANG Ya-ping FU Xin-qiao |
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| Institution: | CHU Qian,WANG Ya-ping,FU Xin-qiao,et al.Department of Neurology,Tongji Hospital,Tongji Medical School of Huazhong University of Science and Technology,Wuhan 430030,Hubei,China |
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| Abstract: | ObjectiveTo study the mechanism of differentiation of mesenchymal stem cells(MSCs) into neuron-like cells in vitro.MethodsMSCs of Wistar rats were separated and cultured, and then induced with DMSO and BHA in vitro. The specific marking proteins of neurons, glia and neural stem cells were detected before preinduction, at 24h after preinduction, at 6h, 24h, and 48h after neuronal induction.ResultsAfter the inducement, many MSCs turned into bipolar,multipolar and taper,and then intersected as network structure. Nestin was strong positive at 6h after neuronal induction, and decreased at 24h, 48h after the induction. NeuN was present at 6 h after neuronal induction, and increased at 24h, 48h after the induction.ConclusionMSCs can be induced into neural stem cells(NSCs) at first, and then differentiate into neuron-like cells in vitro. |
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| Keywords: | mesenchymal stem cells(MSCs) differentiation neuron-like cells neural stem cells(NSCs) |
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