人肝癌细胞和成纤维细胞共育时超微结构的改变

采用定位培养技术 ,将人肝癌细胞和成纤维细胞有序地接种在同一容器内培养 ,电镜下观察 2种细胞接触时细胞切面超微结构的变化。结果 :与肝癌细胞伪足接触的成纤维细胞膜下出现多个吞饮泡 ,使骨架纤维下移 ,局部断裂。 2种细胞的线粒体肿胀 ,溶酶体增多 ,胞质中出现许多泡状结构。提示 ,在肝癌细胞侵袭成纤维细胞早期、成纤维细胞膜尚未受到破坏时 ,细胞内的结构已发生了改变。

Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro

A localized cell culture method in vitro was empolyed to investigate the interaction between human hepatocellular carcinoma cells and fibroblasts. These two kinds of cells were cocultured in the same container by design.To prepare samples for TEM, embedding slice in situ and vertical sectio ning were adopted. It was convenient to observe the ultrastructure of different kinds of cells with loca lized cell culture. The result indicated that mitochondria appeared to be swollen, the number of lysosomes increased in cytoplasm of the two kinds of cells, and microfilaments beneath the cytomembrane of fibroblasts touching the pseudopodia of the tumor cell were pushed down by pinosomes and broken at some areas after being cocultured for 96 h.The result suggests that the interaction between human hepatocellular carcinoma cells and fibroblasts causes changes of cellular structures.