The defectiveness of Mill Hill 2, a carcinoma-inducing avian oncovirus. |
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| Authors: | S S Hu C Moscovici P K Vogt |
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| Institution: | 1. Department of Microbiology, University of Southern California, School of Medicine, 2011 Zonal Avenue, Los Angeles, California 90033, USA;2. Virus Research Laboratory, Veterans Administration Hospital, Gainesville, Florida 32601, USA |
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| Abstract: | The avian carcinoma virus Mill Hill 2 (MH2) transforms fibroblasts and macrophages in tissue culture. It is defective in replication and dependent on a helper virus, MH2 AV, for the production of infectious progeny. MH2 AV contains both subgroup C and A envelope determinants; its helper functions are required by MH2 in the env, pol, and probably also the gag genes. The defects of MH2 can be complemented only by helper viruses of the chicken leukosis group, further suggesting that MH2 is defective in gag or pol, or both. MH2 transformed nonproducer cells synthesize an aberrant viral polyprotein of 100,000 daltons. This MH2p100 carries antigenic determinants of the gag protein p27, but not of the env protein gp85. It is not cleaved into smaller, functional proteins and is not glycosylated. |
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| Keywords: | To whom requests for reprints should be sent |
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