微小小单胞菌与慢性根尖周炎临床症状的相关性研究

目的：研究慢性根尖周炎感染根管中微小小单胞菌( Parvimonas micra,Pm)的检出情况以及与慢性根尖周炎临床症状、体征的关系。方法采集104名慢性根尖周炎患者的120颗患牙根管内标本,其中包括初次治疗组(A组)和再次治疗组(B组)各60颗患牙。利用16S rDNA聚合酶链反应技术检测标本中的微小小单胞菌16S rDNA的表达；用比值比( OR值)的方法,分析Pm检出率与慢性根尖周炎临床症状及体征的关系。结果慢性根尖周炎初次治疗组检出Pm 24例,再次治疗组检出Pm 3例。2组结果通过比值比分析发现,随检出Pm几率增加,慢性根尖周炎根管内出现恶臭(A组OR=3．35,B组OR=14．29)或根尖出现大于5mm的根尖病变(A组OR=2.75, B组OR=6.70)的几率随之增加,95%可信区间下限>1,结果有显著性差异(P<0．05)。结论无论在初次治疗或再次治疗的慢性根尖周炎感染根管中均有Pm 检出。 Pm 可能在该疾病的发生发展过程中发挥一定作用。

Association between Parvimonas micra and the clinical symptoms in chronic apical periodontitis

Objective To investigate the relationship between Parvimonas micra ( Pm) and the clinical symptoms and signs of chronic apical periodontitis. Methods A total of 120 infected teeth from 104 patients with chronic apical periodontitis were collected. Among them, 60 teeth were presented with primary infections ( group A ) and 60 with secondary infections, requiring retreatment (group B). 16S rDNA PCR method was used to detect the expression of Pm 16s rDNA in the samples from the root canal, and odds ratio ( OR) was used to analyze the relationship between the expression of Pm 16s rDNA and the clinical symptoms and signs of chronic apical periodontitis. Results Pm 16s rDNA was expressed in 24 teeth in group A and three in group B. The results of OR showed positive correlations between Pm 16s rDNA expression and the foul smell in the infected root canals ( group A OR =3. 35, group B OR =14. 29 ) and the periapical lesion size greater than 5mm(group A OR=2. 75,group B OR=6. 70). Conclusion Pm could be detected in the root canal with primary infection or secondary infection. Pm might play a role in the occurrence and development of the chronic periapical periodontitis.