高效液相色谱法同时测定复方贯叶金丝桃滴丸中金丝桃苷和黄芩苷的含量

目的:建立同时测定复方贯叶金丝桃滴丸中金丝桃苷和黄芩苷的HPLC方法。方法:采用kromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸(15∶85),检测波长为360 nm,流速1.0 m L/min,柱温30℃。结果:金丝桃苷、黄芩苷分别在0.0815⁰.7335μg(r=0.999 6)、0.12480.7335μg(r=0.999 6)、0.1248¹.1232μg(r=0.999 8)范围内线性关系良好,金丝桃苷的平均加样回收率为99.8%(RSD=0.99%),黄芩苷的平均加样回收率为100.7%(RSD=0.85%)。结论:该方法简便、准确、可靠,可用于贯叶金丝桃滴丸中金丝桃苷、黄芩苷的质量控制。

Simultaneous Determination of Hypericin and Baicalin in Guanye Jinsitao Drop Pills by HPLC

Objective： To develop a method of HPLC for the content determination of Hypericin and Baicalin in Guanye Jinsitao Drop Pills. Methods： The separation temperature was performed at 30 ℃ on a kromasil- C18column（ 4. 6 mm ×250 mm,5 μm） eluted with the mobile phase consisted of acetonitrile- 0. 1% phosphoric acid（ 15 ∶ 85）. The detection wavelength was 360 nm. Results： The calibration curves were linear in the range of 0. 0815- 0. 7335 μg for Hypericin（ r =0. 9996） and the Baicalin was 0. 1248 ~ 1. 1232 μg（ r = 0. 9998）. The average recovery of Hypericin was 99. 8%,（ RSD= 0. 99%） and the Baicalin was 100. 7%（ RSD = 0. 85%）. Conclusion： The method is simple,accurate and replicable,and can be used for the quality control of Hypericin and Baicalin in Guanye Jinsitao Drop Pills.