代谢综合征大鼠痰瘀证与动脉粥样硬化的关系

目的:探讨代谢综合征大鼠痰瘀证与动脉粥样硬化的关系。方法:健康雄性自发性高血压大鼠72只,应用随机数字表随机分为对照组、代谢综合征组和痰瘀型代谢综合征组,每组24只,对照组采用普通饲料喂养,代谢综合征组采用高脂饲料喂养,痰瘀型代谢综合征组采用高脂高糖饲料喂养,实验周期为16周,分别在4、8、16周进行主动脉积分评价及主动脉病理检测。结果:主动脉评分比较:在4、8、16周时,与对照组比较,非痰瘀组和痰瘀组主动脉评分差异有统计学意义(P<0.05)。在8、16周时,与非痰瘀组相比,痰瘀组主动脉评分差异有统计学意义(P<0.05);主动脉病理:在16周时:痰瘀组内膜下可见散在泡沫细胞和平滑肌细胞中度增生,其间可见明显炎症细胞浸润,脂质沉积,中弹力纤维增粗,弹力纤维和胶原纤维排列紊乱;非痰瘀组:大鼠主动脉内膜轻度增厚,小面积斑块,主动脉内膜下可见少量泡沫细胞和平滑肌细胞聚集。结论:内膜泡沫细胞和平滑肌细胞增生、炎症细胞浸润及脂质沉积情况,在痰瘀型代谢综合征组明显高于非痰瘀型代谢综合征组,提示痰瘀与动脉粥样硬化有关。

The Study of the Correlation Betweenbetween Phlegm With with Stagnant BloodB lood Stasis In in Metabolic Syndrome With with Atherosclerosis In in Rats

Objective： To investigate the correlation between phlegm with stagnant bloodblood stasis in metabolic syndrome with atherosclerosis. Methods： 72 male spontaneously hypertensive rats were randomly divided into three groups：control group,MS group and MS with phlegm with stagnant bloodblood stasis group. The control group were feed basic diet,the MS group were feed high- fat- salthigh- fat- salt diet and the MS with phlegm with stagnant bloodblood stasis group were feed Hhigh fat and sugar diet in 16 weeks. And the aortic score and aortic pathology will bewere detected at At2,4,8 and 16 weeks. Results： Comparison of aorta score： the MS with phlegm with stagnant bloodblood stasis group in 4、8、16,8,16 weeks had statistically difference（ P〈0. 05）. Comparison of aortic pathology： in 16 weeks,MS with phlegm with stagnant bloodblood stasis group intimal can be seenhad scattered in the foam cells and smooth muscle cells moderate hyperplasia,during which the visible significant inflammatory cell infiltration,lipid deposition,thickening of elastic fibers,elastic fibers and collagen fibers arranged in disorder; Non- Phlegm phlegm group： rat aortic intimal thickening slightly,small area of the plaque,intima visible under a small amount of foam cells and smooth muscle cells gathered. Conclusion：phlegm Phlegm with stagnant bloodblood stasis has relation with atherosclerosis.