| 脑缺血预处理对沙鼠前脑缺血再灌注后HSP70表达水平及学习记忆能力的影响 |
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| 引用本文： | 李建民,赵雅宁,安超旺,陈长香,朱军,付爱军.脑缺血预处理对沙鼠前脑缺血再灌注后HSP70表达水平及学习记忆能力的影响[J].第二军医大学学报,2010,31(1):55-59. |
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| 作者姓名： | 李建民 赵雅宁 安超旺 陈长香 朱军 付爱军 |
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| 作者单位： | 1. 华北煤炭医学院附属医院神经外科,唐山,063000
2. 华北煤炭医学院护理系,唐山,063000 |
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| 基金项目： | 河北省卫生厅重点课题(07347)~~ |
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| 摘 要： | 目的研究脑缺血预处理对沙鼠前脑缺血再灌注后热休克蛋白70(HSP70)表达水平及动物学习记忆能力的影响。方法100只蒙古沙鼠随机分成假手术(Sham)组、前脑缺血再灌注(I/R)组、脑缺血预处理(IP)组,放线菌酮+脑缺血预处理(Cycloheximide+IP)组,每组25只。后3组参照Kirino法制备前脑缺血动物模型,Kitagawa法制备脑缺血预处理动物模型。再灌注后1、2、3d取各组动物脑组织行H-E染色,观察海马CA1区神经元形态变化;用TUNEL法检测神经细胞凋亡情况;用免疫组织化学和蛋白质印迹法检测HSP70的表达水平。再灌注后3、4、5、6、7d用4-PTT旱路迷宫法对沙鼠的学习记忆能力进行评定,各取均值。结果与Sham组比较,I/R组沙鼠海马CA1区存活神经元密度降低(P<0.05),神经细胞凋亡数量增多(P<0.05),HSP70表达量增加(P<0.05),学习记忆能力下降(P<0.05);与I/R组比较,IP组中沙鼠海马CA1区存活神经元密度增加(P<0.05),凋亡神经细胞数量回降(P<0.05),HSP70表达水平进一步增加(P<0.05),学习记忆能力得到改善(P<0.05);而...
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| 关 键 词： | 缺血预处理 脑缺血 热休克蛋白70 缺血耐受 |
| 收稿时间： | 2008/10/12 0:00:00 |
| 修稿时间： | 2009/12/10 0:00:00 |
Effect of ischemic preconditioning on HSP70 expression and learning/memory after cerebral ischemia-reperfusion in gerbils |
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| LI Jian-min,ZHAO Ya-ning,AN Chao-wang,CHEN Chang-xiang,ZHU Jun,FU Ai-jun.Effect of ischemic preconditioning on HSP70 expression and learning/memory after cerebral ischemia-reperfusion in gerbils[J].Academic Journal of Second Military Medical University,2010,31(1):55-59. |
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| Authors: | LI Jian-min ZHAO Ya-ning AN Chao-wang CHEN Chang-xiang ZHU Jun FU Ai-jun |
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| Institution: | LI Jian-min1,ZHAO Ya-ning2,AN Chao-wang1,CHEN Chang-xiang1,ZHU Jun2,FU Ai-jun1 1. Department of Neurosurgery,Affiliated Hospital of Northern China Coal Medical College,Tangshan 063000,Hebei,China 2. Department of Nursing,Northern China Coal Medical College,China |
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| Abstract: | Objective To study the effect of ischemic preconditioning on heat-shock protein 70 ( HSP70) expression and the learning,memory functions after forebrain ischemia-reperfusion injury in gerbils. Methods Gerbils ( n = 100) were evenly randomized into four groups:Sham group,ischemia -reperfusion( I/R) group,ischemia preconditioning( IP) group,and Cycloheximide + IP group ( Cycloheximide was administered 30 min before IP). Transient forebrain ischemia-reperfusion model was established by bilateral common carotid... |
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| Keywords: | ischemia preconditioning cerebral ischemia heat-shock protein 70 ischemic tolerance |
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