溶石颗粒剂对结石模型大鼠肾骨桥蛋白表达的影响

目的：通过测定用药前后肾脏骨桥蛋白(osteopontin,OPN)的变化,来阐明民间验方溶石颗粒剂防治肾结石的作用机制。方法：采用乙二醇、氯化胺法致大鼠肾结石,同时每天1次ig给予溶石颗粒剂5.8,11.6,17.4 g·kg^-1,实验全程21 d。每7 d作以下测定：尿标本以EDTA法测定钙浓度,比色法测定草酸浓度；肾组织HE染色,以免疫组化法检测大鼠肾骨桥蛋白的表达。结果：模型组大鼠肾OPN的表达明显增加,与溶剂对照组相比P<0.01,其中21 d模型组OPN的表达最高,OPN的表达为溶剂对照组的1.4倍（0.281 3/0.201 8）；溶石颗粒剂各剂量组均能明显抑制大鼠肾结石模型OPN的表达（P<0.05）,且呈现明显的量效关系；溶石颗粒剂各剂量组能减轻大鼠肾脏草酸钙结晶程度,显著降低大鼠尿钙和草酸浓度(P<0.01)。结论：溶石颗粒剂可以抑制大鼠肾结石模型中OPN的表达。

Effect of Rongshi granule on renal stone formation and osteopontin expression in rat urolithiasis model

Objective: To investigate the effect of Rongshi granule on osteopontin(OPN) expression.Method: The urlisthiasis rats were induced by ethylene glycol(EG) and ammonium chloride,the control group rats were non-treated,and the Rongshi granule groups(low-dose group,middle-dose group and high-dose group) were administered Rongshi granule in addition to EG and ammonium chloride in 21 days.Pooled 24 h urine samples from each group were collected weekly with the use of metabolic cages,the concentration of uric calcium and oxalic acid were respectively measured by EDTA and photoelectric colorimetric method.Eight animals from each group were killed at 0,7,14,and 21 days,kidneys were histologic examinaed and immunohistochemical staining.Result: The expression of kidney osteopontin in model group was obviously higher than that of control group(P<0.01),and was up to the highest at 21 days with 1.4 times(0.281 3/0.201 8) of the control group.The expression of kidney osteopontin in all of the Rongshi granule groups were lower than those of model group(P<0.05),with an obvious dose-dependent manner.The degree of the kidney calcium oxalate crystal of the rats in all the Rongshi granule groups was much lower than that of model group,and the uric calcium and oxalic acid were much lower than those of model group(P<0.01).Conclusion: The Rongshi granule could inhibit the expression of osteopontin in rat urolithiasis model.