| 大孔吸附树脂分离纯化钩吻总生物碱的研究 |
| |
| 引用本文： | 赵庆春,郭涛,张萍,张跃新,张利平. 大孔吸附树脂分离纯化钩吻总生物碱的研究[J]. 中国药房, 2006, 17(13): 969-971 |
| |
| 作者姓名： | 赵庆春 郭涛 张萍 张跃新 张利平 |
| |
| 作者单位： | 1. 沈阳军区总医院药剂科,沈阳市,110016
2. 大连医科大学,大连市,116027 |
| |
| 摘 要： | 目的:研究用大孔吸附树脂分离纯化钩吻总生物碱中主要成分的方法和工艺条件。方法:采用不同型号的HPD系列大孔吸附树脂对钩吻总生物碱进行提取纯化,以高效液相色谱法检测钩吻总生物碱中主要成分的峰面积为指标,考察最佳工艺和参数条件。结果:HPD-500、HPD-600、HPD-800型大孔吸附树脂对钩吻总生物碱提取效果好,并得到HPD-800的最佳工艺和参数条件。结论:采用HPD-800型大孔吸附树脂及不同浓度乙醇洗脱的方法,可用于钩吻总生物碱的提取纯化。
|
| 关 键 词： | 钩吻总生物碱 大孔吸附树脂 分离纯化 |
| 文章编号： | 1001-0408(2006)13-0969-03 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005-11-28 |
Study on Separation and Isolation of Total Alkaloids from Gelsemium Elegans Benth.by Macroporous Resin |
| |
| ZHAO Qingchun,GUO Tao,ZHANG Ping,ZHANG Yaoxin,ZHANG Liping. Study on Separation and Isolation of Total Alkaloids from Gelsemium Elegans Benth.by Macroporous Resin[J]. China Pharmacy, 2006, 17(13): 969-971 |
| |
| Authors: | ZHAO Qingchun GUO Tao ZHANG Ping ZHANG Yaoxin ZHANG Liping |
| |
| Affiliation: | 1. Dept.of Pharmacy, The General Hospital ot Shenyang Military Command, Shenyang 110016,China;2. Dalian Medical University,Dalian 116027,China |
| |
| Abstract: | OBJECTIVE:To establish a method and a technical process for the purification of total alkaloids from Gelsemium elegans Benth.with macroporous resin.METHODS:Different types of macroporous resin were used to separate and purify total alkaloids,the HPLC peak areas of the main ingredient of the alkaloids form Gelsemium elegans Benth.were compared and the optimum technological parameters were investigated.RESULTS:The HPD-500,HPD-600,HPD-800type macroporous resin showed better comprehensive absorption property,and HPD-800type macroporous resin were used to investigate parameter.CONCLUSIONS:The method of using HPD-800type and eluting with grade ethanol-water can be used to purify the total alkaloids from Gelsemium elegans Benth.. |
| |
| Keywords: | Total alkaloids from Gelsemium elegans Benth. Macroporous resin Seperation and isolation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
