肝硬变组织内HBV DNA和HBsAg的分布及意义

作者用原位杂交和免疫组织化学PAP法双标记,在51例人肝硬变石蜡组织切片中检测HBV DNA和HBsAg.共检出HBV DNA 21例(41.2％),HBsAg 43例(84.3％),二者同时检出共19例(37.3％).HBVDNA阳性颗粒主要分布于肝细胞的胞浆或同时分布于胞核及胞浆内,少数仅位于核内或沿胞膜内侧分布.51例肝硬变患者中,活动性肝硬变与静止性肝硬变组织中HBV DNA的阳性检出率分别为52.8％和13.3％,HBV DNA和HBsAg二者均阳性的检出率分别为47.2％和13.3％,均以活动性肝硬变为高(P<0.05).结果说明,HBV的感染以及HBV DNA在肝组织中的存在和持续活动是肝硬变发生发展的重要因素之一.

Distribution and significance of HBV DNA and HBsAg in liver cirrhosis

Hepatitis B virus DNA (HBV DNA )and HBsAg were detected in 51 human paraffin - embedded sections of liver cirrhosis by the double labelling technique of in situ hybridization and PAP method . The results showed that the positive sections were 21 (41.2% )for HBV DNA , 43 (84.3% )for HBsAg and 19 (37.3% )for both HBV DNA and HBsAg . HBV DNA - positive granules were localized predominantly in the hepaiocellular cytoplasm or in both nucleus and cytoplasm, a few in the nucleus alone or inner side of the cell membrane . Comparing the active cirrhosis with the inactive cirrhosis , positive rates of HBV DNA were 528% and 13.3% , respectively , and in both HBV DNA and HBsAg they were 47.2% and 13.3%, respectively. The positive rate is higher in active cirrhosis than that in inactive cirrhosis (P<0.05 ). The results indicate that the infection of HBV and the existence and persistent action of HBV DNA in the liver tissues are important factors of the occurrence and development of liver cirrhosis .