高氧、维甲酸对胎鼠肺成纤维细胞c-Jun、c-Fos表达的影响

目的 探讨维甲酸对高氧暴露下胎鼠肺成纤维细胞c—Jun、c—Fos表达的影响。方法 取第2代胎鼠肺成纤维细胞作体外培养，待生长至接近融合状态时，随机分为：空气组，空气 维甲酸组，高氧组，高氧 维甲酸组。于培养30min和1、2、6、12、24、48、72h时，行细胞固定后，应用免疫组化方法捡测c—Jun、c—Fos表达强度并结合计算机图像处理系统进行分析。结果 与空气组相比，高氧1h时c—Jun表达明显增强(P＜0．01)，6—12h时达高峰，以后开始下降，但仍高于正常水平(P＜0．01)；c—Fos的表达在高氧30min即增强(P＜0．01)，2—6h达高峰，以后开始下降，24h后恢复到正常水平。维甲酸能明显下调高氧所诱导的c—Jun、c—Fos高表达，但并不能完全阻止c—Jun、c—Fos的过度表达。结论 c—Jun、c—Fos可能参与了高氧暴露下胎鼠肺成纤维细胞的信号转导过程；维甲酸可部分抑制由高氧所诱导的c—Jun、c—Fos的过度表达。

Effect of Retinoic Acid on the Expression of c-Jun and c-Fos in Hyperoxia in Rat Embryonic Lung Fibroblasts

Objective To study the effect of retinoic acid (RA) on the expression of c Jun and c Fos in hyperoxia in rat embryonic lung fibroblasts. Methods The secondary rat embryonic lung fibroblasts were cultured in vitro. Cells were grown to confluence and then randomly divided into 4 groups: air group, hyperoxia group, air plus RA group, hyperoxia plus RA group. After cells were cultured for 30 min,1 h, 2 h, 6 h, 12 h, 24 h, 48 h, 72 h, the cells were fixed, then c Jun and c Fos were detected by the immunohistochemistry combined with computer based image analysis method. Results As compared with air group, after hyperoxia, the expression levels of c Jun was increased significantly at 1 h ( P <0 01), with a maximum at 6-12 h, and decreased afterward, but still higher than normal level ( P <0 01); The expression of c Fox was increased at 30 min, with a maximum at 2-6 h, then decreased gradually, and returned to the normal level at 24 h. RA could down regulate the expression of c Jun and c Fos induced by hyperoxia remarkably, but not completely blocked the overexpression of c Jun and c Fos. Conclusion c Jun and c Fos might be involved in the signal transduction mediated by hyperoxia in rat embryonic lung fibroblasts; RA could inhibit the overexpression of c Jun and c Fos induced by hyperoxia partially.