肝细胞与肝癌细胞中磷脂酰乙醇胺N-甲基转移酶2表达及活性的比较

目的 比较研究原代培养的大鼠肝细胞与大鼠肝癌细胞内磷脂酰乙醇胺Ｎ－甲基转移酶２（ｐｈｏｓｐｈａｔｉｄｙｌｅｔｈａｎｏｌａｍｉｎｅＮ－ｍｅｔｈｙｌｔｒａｎｓｆｅｒｓａｅ２．ＰＥＭＴ２）表达水平和活性,了解ＰＥＭＴ２与细胞生长、增殖的关系。方法 利用免疫细胞化学及蛋白质免疫印迹方法观察ＰＥＭＴ２蛋白的表达,以「＾３Ｈ－Ｃ 同位素掺入法测定了ＰＥＭＴ２的活性,流式细胞仪分析了两种细胞的细胞周期中ＤＮＡ的

Comparison of the expression and activity of phosphatidylethanolamine N-methyltransferase 2 between primary cultured hepatocytes and hepatoma cells in rats

Objective To comparate the expression and activity of phosphatidyl - ethanolamine N-methyltransferase 2 (PEMT_2) between the primary cultured hepatocytes and CBRH7919 hepatoma cells in rats. Methods The expression and activity of PEMT_2 were demonstrated by immunochemistry, Western immunoblotting and [~3H-CH_3]SAM incorporation. Flow cytometry (FCM) was used to investigate the DNA content of the cells. Results PEMT_2 expression and activity were higher in the primary cultured hepatocytes than in hepatoma cells. Cell cycle analysis showed that the proportion of the hepatocytes was higher in G_1 phase and lower in S phase in the primary cultured hepatocytes than hepatoma cell lines. Conclusion There is an intimate relationship between the expression of PEMT_2 and the proliferation of hepatocytes.