消旋聚乳酸/羟基磷灰石/脱钙骨基质人工骨修复兔桡骨大段骨缺损的实验研究

目的研制理想的,能够修复大段骨缺损的人工骨材料。方法采用乳液共混法将消旋聚乳酸(PDLLA)、羟基磷灰石(HA)、脱钙骨基质(DBM)结合,制成PDLLA/HA/DBM人工骨,并将PDLLA/HA/DBM和PDLLA进行兔桡骨大段骨缺损修复的对比研究。术后2、4、8和12周时摄X片及病理形态学观察及新骨形成定量分析。结果PDLLA/HA/DBM人工骨内新骨形成量明显多于PDLLA及空白对照组(P<0.01),且能够有效修复骨缺损。结论PDLLA/HA/DBM人工骨能促进长骨大段骨缺损的修复,是一种较理想的骨修复材料。

Experimental study of PDLLA/HA/DBM artificial bone for the repair of rabbit radius defect

Objective To prepare an ideal artificial bone material for the repair of segmental defect of long bones. Methods An emulsion blend method was employed to prepare the composite of poly-D-L-lactic acid (PDL-LA), hydroxyapatite (HA) and decalcified bone matrix (DBM), called PDLLA/HA/DBM. PDLLA/HA/DBM and PDLLA were respectively used for the repair of rabbit radius defect, while rabbits with only bone defect were used as the blank control. Plain X-ray examination was performed and specimens of bone and bone repair materials were procured in week 2,4,8 and 12 after operation, respectively. Gross observation, histological observation and qualitative analysis of the new bone formation were performed to compare the outcomes of the two kinds of materials. Results Bone formation rate with PDLLA/HA/DBM was significantly higher than that with PDLLA and that in the blank control. PDLLA/HA/DBM could repair the segmental defect of rabbit radius effectively. Conclusion The PDLLA/HA/ DBM artificial bone can promote the repair of long bone defect and is an ideal artificial material for bone repair.