| HPLC-ELSD法测定肾炎清片中黄芪甲苷的含量 |
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| 引用本文： | 曹红,邢俊波,赵晶. HPLC-ELSD法测定肾炎清片中黄芪甲苷的含量[J]. 中国医药指南, 2013, 0(16): 426-427 |
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| 作者姓名： | 曹红 邢俊波 赵晶 |
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| 作者单位： | 曹红 (总后勤部卫生部药品仪器检验所,北京,100071)； 邢俊波 (总后勤部卫生部药品仪器检验所,北京,100071)； 赵晶 (第二军医大学药学院,上海,200433)； |
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| 摘 要： | 目的建立肾炎清片中黄芪甲苷的含量测定方法。方法采用HPLC-ELSD法测定肾炎清片中黄芪甲苷的含量;色谱柱为AgilentZORBAX Eclipse XDB-C18(4.6mm×150mm,5μm);流动相为乙腈-水(35∶65);流速为1.0mL/min;柱温为40℃。结果黄芪甲苷在0.4004～4.004mg线性关系良好(r=0.9999),平均回收率为96.11%(RSD=1.14%)。结论本方法结果准确可靠,重复性好,操作简便。
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| 关 键 词： | 黄芪甲苷 肾炎清片 HPLC–ELSD |
Determination of Astragaloside IV in Shenyanqing Tabletes by HPLC -ELSD |
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| CAO Hong,XING Jun-bo,ZHAO Jing. Determination of Astragaloside IV in Shenyanqing Tabletes by HPLC -ELSD[J]. Guide of China Medicine, 2013, 0(16): 426-427 |
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| Authors: | CAO Hong XING Jun-bo ZHAO Jing |
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| Affiliation: | CAO Hong, XING Jun-bo, ZHAO ding2 ( 1 General Logistics Department of the Ministry of Health Drug and Instrument Control, Beijing 100071; 2 College of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
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| Abstract: | Objetive To establish a method for the determination of astragaloside IV in Shenyanqing Tabletes. Mehhod HPLC -ELSD method was developed for the determination of astragaloside IV in Shenyanqing Yabletes. The chromatographic column was Agilent ZORBAX Eclipse XDB-C18 （4.6mm×150mm, 5pro） ; The mobile phase was acetonitrile- H20 （35 ： 65） with a flow rate of 1.0 ml/min. Results astragaloside IVshowed a good linear relationship within the range of 0.4004-4.004mg, （r=0.9999） . the average recovery was 96.11% （RSD =1.14%） . Conclusion This method is reliable and simple with good repeatability. |
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| Keywords: | Astragaloside IV Shenyanqing Tabletes Quantitative analysis HPLC ELSD |
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