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| 鲑鱼鱼白DNA对Balb/c小鼠胸腺基因表达的影响 | |
| 引用本文： | 程民,杜伯雨,陈文华,车会莲,周令望,李蓉. 鲑鱼鱼白DNA对Balb/c小鼠胸腺基因表达的影响[J]. 免疫学杂志, 2005, 21(1): 40-43 |
| 作者姓名： | 程民 杜伯雨 陈文华 车会莲 周令望 李蓉 |
| 作者单位： | 1. 哈尔滨医科大学公共卫生学院卫生检验学教研室,哈尔滨,150001 2. 大连医科大学卫生学教研室,辽宁,大连,116027 3. 哈尔滨医科大学克山病防治所,哈尔滨,150086 |
| 基金项目： | 国家自然科学基金资助项目 (30 0 70 656) |
| 摘 要： | 目的探讨鲑鱼鱼白DNA(Salmon milt DNA，SMD)对小鼠胸腺增龄性萎缩作用的分子机理。方法10月龄雌性Balb／c小鼠按体质量随机分为实验组和对照组，在常规饮食的基础上各组每日分别灌胃333．33和0mg／kg的SMD，5周后，无菌取胸腺；应用RT-PCR和Northern blot技术检测Genebank登录号为Aw209102、U23789、X80232、NM_009678和AF083929的基因在对照组和实验组胸腺组织中的表达情况。结果Aw209102、U23789、X80232在实验组表达上调；NM_009678、AF083929在实验组表达下调。结论SMD可通过促进细胞增殖相关基因和发育、分化相关基因表达，并抑制细胞凋亡相关基因的表达而逆转胸腺增龄性萎缩。 |
| 关 键 词： | 鱼鱼白DNA 小鼠 胸腺 逆转录聚合酶链式反应 Northern blot |
| 文章编号： | 1000-8861(2005)01-0040-04 |
| 修稿时间： | 2003-11-03 |
Effects of salmon milt DNA on the gene expression in thymus of old Balb/c mice |
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| CHENG Min ,DU Bo-yu ,CHEN Wen-hua ,CHE Hui-lian ,ZHOU Ling-wang ,LI Rong. Effects of salmon milt DNA on the gene expression in thymus of old Balb/c mice[J]. Immunological Journal, 2005, 21(1): 40-43 | |
| Authors: | CHENG Min DU Bo-yu CHEN Wen-hua CHE Hui-lian ZHOU Ling-wang LI Rong |
| Affiliation: | CHENG Min 1,DU Bo-yu 2,CHEN Wen-hua 1,CHE Hui-lian 1,ZHOU Ling-wang 3,LI Rong 1 |
| Abstract: | Objective To investigate the molecular mechanism of salmon milt DNA (SMD) in age-related involution of mouse thymus. Methods Ten-month-old female Balb/c mice were divided randomly into experiment and control groups according to their weights. The mice in the experiment group were supplied with SMD (333.33 mg/kg) but the mice in the control group were not supplied with SMD. RT-PCR and Northern blot were used to detect the expressions of Aw209102, U23789, X80232, NM009678, and AF083929 genes in all their thymuses. Results The expression levels of Aw209102, U23789, and X80232 genes were higher in experiment group those in control group, but the expression levels of NM009678 and AF083929 genes were lower in experiment group than in control group. Conclusion SMD may reverse the age-related involutions in mouse thymus via up-regulating the expression of proliferation related genes as well as the expression of development and differentiation related genes, and via down-regulating the expression of apoptosis related genes simultaneously. |
| Keywords: | Salmon milt DNA Mice Thymus RT-PCR Northern blot |
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