bcr rearrangement and C-abl gene expression in Ph1-positive hybrid acute leukemia with simultaneous proliferation of lymphoid and myeloid blasts |
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| Authors: | Mihiro Okabe Makoto Oita Yasuuki Kunieda Jun Matsuura Keisuke Sakurada Shozo Matsushima Mitsuaki Kakinuma Tamotsu Miyazaki |
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| Affiliation: | (1) Third Department of Internal Medicine, Hokkaido University School of Medicine, Nishi-7, Kita-15, Kita-ku, 060 Sapporo, Japan;(2) Section of Genetics, National Cancer Center Research Institute, Tokyo, Japan;(3) Section of Bacterial Infection, Institute of Immunological Science, Hokkaido University, Sapporo, Japan |
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| Abstract: | Summary bcr gene rearrangement and c-abl gene expression were analyzed in a patient with Philadelphia chromosome (Ph1)-positive hybrid acute leukemia with simultaneous proliferation of lymphoid and myeloid blasts. These data were compared with those from a patient with chronic myelogenous leukemia (CML) in mixed crisis. The leukemic cells of both patients showed immuno-phenotypic profiles such as non-T, non-B common ALL with some MPO-positive leukemic cells and rearranged JH genes. On analysis of molecular events associated with the Ph1 chromosome, the leukemic cells of a patient with CML in mixed crisis showed bcr rearrangement and an 8.5-kb bcr-abl chimeric mRNA, but those of a patient with Ph1-positive hybrid acute leukemia showed no 8.5-kb bcr-abl mRNA, as previously reported in a number of Ph1-positive acute lymphoblastic leukemia (ALL) cases. These results revealed that the molecular event found in Ph1-positive ALL is not only restricted to lymphoid lineage but may play an important role in the proliferation of the myeloid lineage. |
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| Keywords: | Hybrid acute leukemia Ph1 chromosome bcr Gene Immunoglobulin JH gene |
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