| 上海市罕见沙门菌型腹泻的溯源研究 |
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| 引用本文： | 许学斌,顾宝柯,冉陆,刁保卫,陈敏,金汇明,肖文佳,袁政安.上海市罕见沙门菌型腹泻的溯源研究[J].上海预防医学,2009,21(1):7-10. |
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| 作者姓名： | 许学斌 顾宝柯 冉陆 刁保卫 陈敏 金汇明 肖文佳 袁政安 |
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| 作者单位： | 1. 上海市疾病预防控制中心,上海,200336
2. 中国疾病预防控制中心,北京,100050 |
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| 基金项目： | ，中美新发和再发传染病合作项目 |
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| 摘 要： | 目的]初步了解本市罕见沙门菌血清型菌株的分子流行病学特征。方法]对分离自不同来源标本的22株7种不同血清型沙门菌进行脉冲凝胶电泳(PFGE)分析,脉冲凝胶电泳选用XbaⅠ限制性内切酶。结果]2株列克星敦、2株非丁伏斯沙门菌的电泳图谱显示有较大差异,2株蒙得维的亚、2株朗根萨尔查、2株乌普萨拉、5株胥戈成格隆、4株波摩那的图谱显示存在同源性。结论]本市沙门菌罕见血清型的流行菌株和食品菌株之间既存在分子水平的同源性也存在非同源性,加强沙门菌综合监测和即时分子分型可预警罕见沙门菌血清型流行。
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| 关 键 词： | 沙门菌 罕见血清型 脉冲凝胶电泳 分子分型 |
Sourcing reseach on rare serotype Salmonella diarhea in Shanghai |
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| XU Xue-bin,GU Bao-ke,RAN Lu,DIAO Bao-wei,CHEN Min,JIN Hui-ming,XIAO Wen-jia,YUAN Zheng-an.Sourcing reseach on rare serotype Salmonella diarhea in Shanghai[J].Shanghai Journal of Preventive Medicine,2009,21(1):7-10. |
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| Authors: | XU Xue-bin GU Bao-ke RAN Lu DIAO Bao-wei CHEN Min JIN Hui-ming XIAO Wen-jia YUAN Zheng-an |
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| Institution: | 1.Shangahi Municipal Center of Disease Control and Prevention;Shanghai 200336;China;2.China Center of Disease Control and Prevention;Beijing 100050;China |
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| Abstract: | Objective] To understand the molecular epidemiological characteristics of some rare serotype Salmonella with pulsed field gel electrophoresis(PFGE) in Shanghai. Methods] 22 strains of Salmonella including seven serotypes were isolated from different sources.PFGE were performed by genomic DNA digestion with Xba I restriction enzyme.Results] Significant difference were expressed between two strains of Salmonella Lexington and two strains of Salmonella Hvittingfoss in electrophoretogram map.Homology were ex... |
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| Keywords: | Salmonella Rare serotype Pulsed field gel electrophoresis Molecular typing |
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