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The Diverse Actions of Volatile and Gaseous Anesthetics on Human-cloned 5-Hydroxytryptamine3 Receptors Expressed in Xenopus Oocytes |
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| Authors: | Suzuki Takahiro MD; Koyama Hideki BS&#x;; Sugimoto Masahiro MD; Uchida Ichiro MD PhD&#x;; Mashimo Takashi MD PhD |
| Institution: | Suzuki, Takahiro M.D.*; Koyama, Hideki B.S.†; Sugimoto, Masahiro M.D.*; Uchida, Ichiro M.D., Ph.D.‡; Mashimo, Takashi M.D., Ph.D.§ |
| Abstract: | Background: General anesthetics can modulate the 5-hydroxytryptamine type 3 (5-HT3) receptor, which may be involved in processes mediating nausea and vomiting, and peripheral nociception. The effects of the new volatile anesthetic sevoflurane and the gaseous anesthetics nitrous oxide (N2O) and xenon (Xe) on the 5-HT3 receptor have not been well-characterized. Methods: Homomeric human-cloned 5-HT3A receptors were expressed in Xenopus oocytes. The effects of halothane, isoflurane, sevoflurane, N2O, and Xe on 5-HT-induced currents were studied using a two-electrode, voltage clamping technique. Results: Halothane (1%) and isoflurane (1%) potentiated 1 mu]m 5-HT-induced currents to 182 +/- 12 and 117 +/- 2%, respectively. In contrast, sevoflurane (1%), N2O (70%), and Xe (70%) inhibited 5-HT-induced currents to 76 +/- 1, 77 +/- 4, and 34 +/- 4%, respectively. The inhibitory effects were noncompetitive for sevoflurane and competitive for N2O and Xe. None of these inhibitory effects showed voltage dependency. |
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