| 毕赤酵母工程菌高密度发酵肿瘤坏死因子相关凋亡诱导配体的研究 |
| |
| 引用本文： | 李继东,林跃鑫,李茹冰,佟明华,万华印,孔祥平. 毕赤酵母工程菌高密度发酵肿瘤坏死因子相关凋亡诱导配体的研究[J]. 中国生化药物杂志, 2004, 25(1): 23-25 |
| |
| 作者姓名： | 李继东 林跃鑫 李茹冰 佟明华 万华印 孔祥平 |
| |
| 作者单位： | 1. 福建师范大学,生物工程学院,福建,福州,350007
2. 中国人民解放军458医院全军传染病中心分子生物学科,广东,广州,510602 |
| |
| 摘 要： | 目的研究重组人可溶性肿瘤坏死因子相关凋亡诱导配体 (TRAIL)巴斯德毕赤酵母工程菌发酵工艺。方法首先用摇瓶对工程菌的甲醇诱导周期、甲醇浓度、pH进行研究 ,在此基础上 ,采用 5L发酵罐补料培养进行中试研究。结果人可溶性TRAIL巴斯德毕赤酵母菌在pH 6 .0、甲醇诱导浓度 1%的条件下诱导 96h ,目标蛋白质浓度最高 ,可达 72 .8mg/L。 5L发酵罐培养放大 ,可以达到 184mg/L。结论此发酵工艺可以较好地提高人可溶性TRAIL工程菌的分泌表达。
|
| 关 键 词： | 重组人肿瘤坏死因子相关凋亡诱导配体 毕赤酵母 发酵 |
| 文章编号： | 1005-1678(2004)01-0023-03 |
| 修稿时间： | 2003-01-27 |
Study on high density fermentation of recombinant Pichia pastoris expressing recombinant humace soluble TNF-related apoptosis-inducing ligand |
| |
| LI Ji-dong ,LIN Yue-xin ,LI Ru-bing ,TONG Ming-hua ,WAN Huan-yin ,KONG Xiang-ping. Study on high density fermentation of recombinant Pichia pastoris expressing recombinant humace soluble TNF-related apoptosis-inducing ligand[J]. Chinese Journal of Biochemical Pharmaceutics, 2004, 25(1): 23-25 |
| |
| Authors: | LI Ji-dong LIN Yue-xin LI Ru-bing TONG Ming-hua WAN Huan-yin KONG Xiang-ping |
| |
| Affiliation: | LI Ji-dong 1,LIN Yue-xin 1,LI Ru-bing 2,TONG Ming-hua 2,WAN Huan-yin 2,KONG Xiang-ping 2 |
| |
| Abstract: | PurposeTo study influential factors of the high density fermentation of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) Pichia pastoris.MethodsThe effects of different methanol inducement period, methanol concentration,pH were observed using shaking flask, and fed-batch fermentor was also studied.Results72.8 mg/L rhsTRAIL was obtained at pH 6.0,1.0% methanol on the fourth day and 184 mg/L rhsTRAIL were obtained in fed-batch fermentor.ConclusionThe developed fermentative technology might incease the expression of sTRAIL in recombinant Pichia pastoris strain. |
| |
| Keywords: | recombinant human soluble TNF-related apoptosis-inducing ligand Pichia pastoris fermentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
