间充质干细胞对不同刺激条件下Th1/Th2细胞比值的影响

目的探讨间充质干细胞(MSC)对不同刺激作用下T淋巴细胞向Th1及Th2亚群转化的影响。方法将MSC植入24孔板中,在第3天加入经佛波脂(PMA)及离子霉素(ionomyc in)刺激的T淋巴细胞,或者按照不同比例将MSC加入双向混合淋巴细胞培养体系中,用流式细胞术分析各组T淋巴细胞亚群和内因子IL-4、IFN-γ的分泌变化情况,从而间接了解Th1及Th2亚群的改变。结果对于共刺激作用下单独培养的T细胞,MSC能轻度抑制其Th1细胞的转化;而在混合淋巴细胞培养体系(MLC)中,MSC能够明显抑制其中的CD8 T细胞亚群和Th1细胞,轻度提高Th2细胞比率。结论MSC在体外能抑制CD8 T细胞(CTL)和Th1细胞,升高Th2细胞。所以在临床上可能有减轻移植后急性移植物抗宿主病(aGVHD)的发生,而保留移植物抗白血病(GVL)的潜力。

Effect of mesenchymal stem cell on the rate of Th1/Th2 with different stimulations

Objective To investigate the effect of mesenchymal stem cell(MSC) on the balance of Th1/ Th2 with different stimulations.Methods To inoculate MSC in 24-well tissue culture plates.after 3 days, added T lymphocytes co-stimulated by PMA and Ionomycin,or the MSC were added to the two-way mixed lymphocyte culture according to different proportion.The subsets and cytokines of T lymphocytes were analyzed by flow cytomety.Results Differentiation into Th1 of T lymphocytes activated by co-stimulation can be inhibited MSC;In MLC,CD8~ T cell subsets and Th1 were evidently decreased.But Th2 cells were slightly increased.Conclusion MSC can significantly suppress CD8~( )T cell,Th1,increase Th2.MSC has potentialities of alleviating acute graft-versus-host disease(aGVHD) and maintaining graft versus leukemia(GVL).