大鼠心肾阳虚证动物模型实验研究

目的　心肾阳虚证是中医临床上一种常见的病证 ,本实验研究欲建立与临床相应的大鼠心肾阳虚证动物模型。方法　从对中医心肾阳虚证的模拟入手 ,依据相关中医理论及心肾阳虚证的症状、体征 ,将大鼠甲状腺切除并 ip阿霉素 ,从而造成大鼠心肾阳虚模型。结果　大鼠甲状腺切除并 ip阿霉素后 ,动物左心室功能明显减退且甲状腺功能显著性低下 ,以及伴畏寒、蜷缩、呼吸微弱等 ,出现了非常类似人类心肾阳虚证的主要症状 ,认为该方法可造成大鼠心肾阳虚模型。结论　大鼠甲状腺切除并 ip阿霉素后 ,动物的症状、体征能体现所模拟的心肾阳虚证型 ,可将该方法作为中医心肾阳虚证动物模型的制作方案

Experimental Study on Animal Model with Yang Deficency of Heart and Kidney in Rats

Objective Yang deficiency of heart and kidney is a high incidence syndrome in clinical practice of TCM.We hope to produce the animal model of this syndrome in rat, corresponding to the human's similar groups of symptoms in clinical practice.Method According to the correlated theory of TCM, the clinical syndrome and physical sign of Yang deficiency of heart and kidney,We simulated this syndrome by cutting rat's thyroid and injecting(i.p.) doxorubicin. Result After cut thyroid and injected(i.p.) doxorubicin, Rats showed a functional decline of left ventricle and thyroid, accompanied by some symptoms like cold fearing,weary ,feeble inhale and exhale, etc. This syndrome was very similar to the syndrome of Yang defciency of heart and kidney of human. So,it was believed that we can reproduce this syndrome of human in rats by this method. Conclusion The syndrome that rats showed after cutting thyroid and injecting(i.p.) doxorubicin can represent the Yang deficiency of heart and kidney of human. And this method can be used to simulate the human's syndrome of Yang deficiency of heart and kidney.