IL-6对培养PVR的视网膜色素上皮细胞生长的影响及地塞米松的调节作用

目的　探讨白细胞介素 6(IL 6)对增生性玻璃体视网膜病变 (PVR)的视网膜色素上皮 (RPE)细胞体外生长的影响 ,以及地塞米松对这种增生的调节作用。方法　将地塞米松加入体积比为 10 %PVR的玻璃体切割物培养的RPE细胞培养板中 ,放射免疫学方法检测IL 6的分泌 ;将IL 6、PVR的玻璃体切割物和RPE细胞共同培养 ,测定掺入的3 H TdR和3 H proline的cpm值 ;用不同浓度的地塞米松、PVR玻璃体切割物和RPE细胞共同培养 ,测定掺入的 3 H TdR和3 H proline的cpm的值。 结果　加入地塞米松培养PVR的RPE细胞合成IL 6的量减少 ;IL 6和PVR的RPE细胞共同孵育后 ,细胞增殖明显 ,胶原合成增强 ;地塞米松抑制培养PVR的RPE细胞增殖和胶原合成 ,且随浓度的增加 ,抑制作用增强。结论　IL 6促进PVR培养的RPE细胞增殖和胶原的合成 ,地塞米松抑制RPE细胞增殖和胶原合成 ,一定程度是通过抑制IL 6合成而发挥作用。

Effect of interleukin-6 on the proliferation of RPE cells and the regulation of dexamethasome

ObjectiveTo investigate the effect of IL-6 on the proliferation of retinal pigment epithelial (RPE) cells and the regulation of dexamethasome(Dex).MethodsRPE cells were cultivated using 10%(V/V) extraction of proliferative vitreoretinopathy (PVR) and Dex.The levels of IL-6 were determined by radioimmunoassay.The cpm values of 3H-TdR and 3H-proline from the RPE cells cultivated with 10% PVR,IL-6 and Dex of different concentrations were determined respectively.ResultsThe production of IL-6 from RPE cells cultivated by PVR and Dex was reduced.The values of 3H-TdR and 3H-proline of the RPE cells cultivated by PVR with IL-6 were higher than those by PVR along,from which the values of 3H-TdR and 3H-proline of the RPE cells were higher than those cultivated by fatal bovine serum(FBS).The values 3H-TdR and 3H-proline of the RPE cells cultivated by PVR and Dex were lower than those by PVR.The values of 3H-TdR and 3H-proline were reduced with increase of Dex concentration.ConclusionIL-6 promotes the proliferation of RPE cells cultivated by PVR,but Dex reduces the proliferation through the inhibition toward the production of IL-6 form RPE cells with PVR in part.