肉桂醛氧氟沙星酰腙诱导人胰腺癌细胞凋亡的实验研究

 目的 探讨肉桂醛氧氟沙星酰腙N-(3-苯亚丙烯基)-6-氟-1， 8-(2， 1-丙氧基)-7- (4-甲基哌嗪-1-基)-喹啉-4 (1H)-酮-3-甲酰肼，肉桂醛氧氟沙星酰腙]诱导人胰腺癌BxPC-3细胞凋亡的作用及机制。方法 用不同浓度的肉桂醛氧氟沙星酰腙处理BxPC-3细胞，采用四甲基偶氮唑蓝 (MTT)比色法，检测肉桂醛氧氟沙星酰腙对BxPC-3细胞的增殖抑制效应；采用形态学、TUNEL法和DNA琼脂糖凝胶电泳，检测细胞凋亡的发生；应用蛋白印迹技术检测凋亡相关基因 caspase-3，caspase-8，caspase-9，bcl-2，bax和细胞色素C的表达。结果 肉桂醛氧氟沙星酰腙在体外对BXPC-3细胞有显著增殖抑制效应，且呈浓度、时间依赖关系；在肉桂醛氧氟沙星酰腙作用下，BxPC-3细胞出现显著的细胞凋亡征象；Hoechst33258/PI荧光染色显示细胞固缩，核染色质聚集或断裂，形成凋亡小体；TUNEL实验统计细胞凋亡率显著高于对照组（P

Cinnamaldehyde Ofloxacin-3-Ylhydrazone Induces Apoptosis of Human Pancreatic Carcinoma Cells

OBJECTIVE To study the effect of cinnamaldehyde ofloxacin-3-ylhydrazone (FQ16) on apoptosis of human pancreatic carcinoma cells in vitro. METHODS Human pancreatic carcinoma cell line BxPC-3 was treated with FQ16 at different concentrations. The proliferation inhibition was examined by MTT assay. Morphological examination， TUNEL and agarose gel electrophoresis method were used to detect apoptosis. Western blotting method was used to detect the expression of apoptosis related genes caspase-3， caspase-9， caspase-8， bcl-2， bax and cytochrome C. RESULTS The cell proliferation was inhibited by FQ16 in a time- and a dose-dependent manner. Treatment of BxPC-3 cells with different concentrations of FQ16 for 24 h increased the percentage of the apoptotic cells obviously (P