七氟烷短时吸入对新生大鼠海马caspase-3活性和认知功能发育的影响

目的探讨七氟烷短时吸入对新生大鼠海马caspase-3活性和认知功能发育的影响。方法 7日龄新生SD大鼠64只,随机分为A、B、C、D 4组(n=16):A组吸入40%氧气1 h,B、C、D组分别吸入1.0%、2.0%、4.0%七氟烷1 h。各组随机取8只大鼠,于吸入七氟烷后24 h采用流式细胞仪定量分析海马caspase-3的活性,观察海马神经细胞凋亡情况;各组余8只大鼠于7 wk采用Morris水迷宫测试其认知功能。结果 B、C、D组Morris水迷宫测试成绩低于A组,而且随着吸入七氟烷浓度的增加,逃避潜伏期和探索时间逐渐延长,差异有统计学意义(均P<0.01);吸入1.0%、2.0%、4.0%七氟烷大鼠海马神经细胞凋亡率分别为(31.0±2.7)%、(52.8±4.9)%、(71.4±3.2)%,均高于A组(1.9±0.8)%,而且随着吸入七氟烷浓度的增加,神经细胞凋亡率逐渐增加,差异有统计学意义(均P<0.01)。结论七氟烷短时吸入导致海马神经细胞凋亡,并阻抑新生大鼠认知功能发育。

Effects of short-term exposure to sevoflurane on cognitive function development and hippocampal caspase-3 activation in neonatal rats

AIM To investigate the effects of short-term exposure to sevoflurane on cognitive function development and hippocampal caspase-3 activation in neonatal rats.METHODS Sixty-four 7-day-old neonatal Sprague Dawley rats were randomly divided into four groups(16 rats in each group).Rats in the group A,B, C and D were respectively inhaled with 40%oxygen,1.0%,2.0%and 4.0%sevoflurane for 1 hour.Eight rats in each group were randomly subjected to hidden platform trails and probe trails in Morris water maze to access cognitive function at age of 7 weeks after anesthetic exposure to sevoflurane.Twenty-four hours after completion of sevoflurane exposure,the neurodegeneration and the activation of caspase-3 in hippocampus were analyzed by flow cytometry.RESULTS Latencies and probe times of group B,C and D were significantly longer than those of group A,which raising with the increasing concentrations of sevoflurane exposure(P<0.01).The ratios of neurodegeneration in hippocampus of group B,C and D were(31.0±2.7)%,(52.8±4.9)%,(71.4±3.2)%, and were significantly higher than those of group A.The ratios of neurodegeneration were in accordance with the increasing concentrations of sevoflurane(P<0.01).CONCLUSION Short-term exposure to sevoflurane may interfere the development of cognitive function and induce hippocampal neurodegeneration in neonatal rats.