胰岛素受体酪氨酸蛋白激酶在重度烫伤大鼠胰岛素抵抗中作用的研究

以３０％体表面积背部皮肤全层烫伤大鼠为模型，通过建立肝细胞膜和骨骼肌细胞膜外源性底物磷酸化的分析方法，观察烫伤后３ｄ胰岛素刺激的胰岛素受体酪氨酸蛋白激酶海性的改变，探讨创伤后胰岛素抵抗的分子机理。

Effects of insulin receptor tyrosine protein kinase on insulin resistance after scalding in rats

After the rats were inflicted with 30%TBSA full thickness scalding on the back,the changes of insulin receptor tyrosine protein kinase(IRTPK)were studied on the 3rd day postscalding with the phosphorylation of external substrates stimulated with hepatic and muscular cytomembranes to determine the possible molecular mechanism of insulin resistance after scalding.It was found that insulin resistance appeared in the early stage postscalding while the basic value of the phosphorylation of external substrates stimulated with hepatic and muscular cytomembrane was increased and the activity of IRTPK to insulin was greatly suppressed as compared with the control.These facts indicate that the imparied responses of IRTPK to the stimulation of insulin may affect down-stream signal transmission of the insulin receptors and then the signal coupling pathway for the glucose transmembranous transportation and glycogen synthesis mediated with IRTPK and stimulated with insulin is obstructed.Consequently,insulin resistance occurs.