Ras和p16基因在肝癌组织中的表达与临床意义

目的：观察基因ras和p16在肝癌组织中的表达，探讨其在肝癌术后复发及预后中的作用。方法：应用免疫组化S－P法，检测53例手术切除肝癌组织和12例正常肝组织的ras和p16表达及与肝癌临床病理指标、术后复发及预后的关系。结果：（1）ras在肝癌和正常肝组织中的阳性表达率分别为66．0％和34．0％，ras表达与肿瘤大小、有无肝内转移密切相关（P均＜0．05）。ras阳性、阴性表达病例3年复发率分别为68．8％、38．9％，3年生存率分别为37．1％、66．7％（P＜0．05）。（2）p16在肝癌和正常肝组织中的阳性表达率各为47．2％、100．0％，p16表达与有无肝内转移、组织分化密切相关（P均＜0．05）。p16阳性、阴性表达病例3年复发率分别为48．0％、67．9％，其3年生存率分别为64．0％、32．1％（P均＜0．05）。（3）ras和p16在肝癌中的表达密切相关（P＜0．01），p16阳性ras阴性与p16阴性ras阳性表达病 列3年复发率分别为35．7％、79．2％，3年生存率分别为71．4％、29．2％，差异有极显著性（P＜0．01）。结论：联合检测p16和ras的表达可作为判断肝癌术后复发及预后的有效指标，多基因分析比单基因分析更有价值。

CLINICAL SIGNIFICANCE OF RAS AND P16 PROTEIN EXPRESSION IN HEPATOCELLULAR CARCINOMA

Objective:To approach ras and p16 protein expression in hepatocellular carcinoma .To investigate their effect on recurrence and prognosis of hepatocellular carcinoma after hepatectomy.Methoeds:Ras and p16 protein expression in 53 cases of human hepatocellular carcinoma and in 12 cases of normal liver were examined by using immunohistochemical technique,respectively.Results:(1)Positive staining rate of ras in hepatocellular carcinoma and in normal liver was 66.0% and 34.0%(P<0.05),respectively.The positive expression of ras correlated with tumor size,intrahepatic metastsis,recurrence and survical rate after hepatectomy(P<0.05).(2)Positive staining rate of p16 was more higher in normal liver than in hepatocellular carcinoma(P<0.01).The negative expression of p16 correlated with phthological grading ,intrahepatic metastasis and prognosis(P<0.05).(3)There was a significant correlation between ras and p16 protein expression (P<0.01).The 3-year recurrence and survival rate was 35.7% and 71.4% respectively in the opposites.Conclusions: Protein expressions of ras and p16 are effective in dicators for recurrence and prognosis of hepatocellular carcinoma.The analysis of multi-gene is more valuable than that of single-gene.