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Increased SPHK1 expression is associated with poor prognosis in bladder cancer |
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| Authors: | Xiao-Dong Meng Zhan-Song Zhou Jian-Hong Qiu Wen-Hao Shen Qu Wu Jun Xiao |
| Affiliation: | 1. Department of Urology, Southwest Hospital, The Third Military Medical University, No. 30, Gaotanyanzheng Street, Shapingba District, Chongqing, 40038, China 2. Department of Urology, The Bethune International Peace Hospital, No. 398, Zhongshan West Road, Shijiazhuang, 050082, China 3. The Bethune International Peace Hospital, No. 398, Zhongshan West Road, Shijiazhuang, 050082, China 4. Department of Anesthesiology, The Bethune International Peace Hospital, No. 398, Zhongshan West Road, Shijiazhuang, 050082, China |
| Abstract: | Upregulation of sphingosine kinase 1 (SPHK1) protein has been reported to be associated with a poor prognosis in a variety of malignant tumors. However, the role of SPHK1 in bladder cancer (BC) has not been thoroughly elucidated. The purpose of this study was to assess SPHK1 expression and to explore its contribution to BC. Real-time quantitative reverse transcriptase–polymerase chain reaction (qRT-PCR) was conducted to detect SPHK1 mRNA expression in 37 pairs of fresh-frozen BC tissues and corresponding noncancerous tissues. Results showed that SPHK1 mRNA expression level in BC tissues was significantly higher than that in corresponding noncancerous tissues. To investigate the association between SPHK1 protein expression and clinicopathological characteristics of BC, immunohistochemistry (IHC) was performed in 153 archived paraffin-embedded BC samples. Interestingly, high SPHK1 expression was significantly associated with histologic grade (P?=?0.045) and tumor stage (P?P? |
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