安徽地区皖籍汉族人群HLA-G基因多态性

目的探讨安徽地区汉族人群人类白细胞抗原G(HLA-G)基因多态性,并与其他种族人群进行比较.方法采用多聚酶链反应-限制性酶切片段长度多态性(PCR-RFLP)法分析141例皖籍汉人HLA-G等位基因及基因型频率.结果安徽地区汉族人HLA-G等位基因频率以HLA-G*01012(48.5%)最高,G*01011(38.7%)次之,二者共占87.2%,HLA-G*01013(12.8%)较少,未发现HLA-G*0103等位基因.HLA-G基因型分布以HLA-G*01011/01012最高,其他依次为HLA-G*01012/01012＞G*01011/01011＞G*01012/01013＞G*01011-01013,G*01013/01013频率最低.HLA-G等位基因分布在不同地区不同种族人群存在明显差异.与其他种族比较的结果表明,HLA-G*01011在芬兰人、西班牙人中所占比例较高,而中国人、日本人、葡萄牙白人则以HLA-G*01012最高,仅葡萄牙白人中存在HLA-G*0103,但频率较低(占1.2%).结论安徽地区汉族人HLA-G基因多态性与其他地区、种族人群存在差别.

Polymorphism of HLA-G gene in Chinese Hans in Anhui Area

Objective To study polymorphism of HLA - G gene in Chinese Hans in Anhui area and to explore difference from other races.Methods HLA - G allelic frequencies and genotype frequencies were analyzed in 141 randomly selected, healthy, unrelated Chinese individuals by PCR- RFLP method.Results The following allelic frequencies were calculated in Chinese Hans: HLA-G* 01012 = 0.485; G* 01011 =0.387; G* 01013 = 0.0128; HLA-G* 0103 was not found in this group.Distribution of HLA-G genotypes based on alleles HLA - G * 01011, G * 01012 and G * 01013 included HLA - G * 01011/G * 01011 = 0.135; G * 01012/ G* 01012 = 0.227; G* 01013/G* 01013 = 0.021; G* 01011/G* 01012 = 0.404; G* 01012/G* 01013 = 0.113; G*01011/G * 01013 = 0.099. Compared with other races, frequency of HLA - G * 01011 was the same as that in Finnish and Spanish population, and frequency of HLA - G * 01012 was the same as that in Japanese and Portuguese population.HLA - G * 0103 was found only in. Portuguese Whites. Conclusions Polymorphism of HLA - G gene in Chinese Hans is different from that in population of other races.