清香散对湿热证大鼠的抗氧化和肝细胞凋亡作用

目的：观察湿热证大鼠模型的氧化损伤和肝细胞凋亡情况,以及清香散对湿热证大鼠的抗氧化和肝细胞凋亡的作用。方法：Wistar大鼠随机分为湿热证模型组、清香散治疗组和正常对照组,采用流式细胞术分析和电镜检测肝细胞凋亡改变,同时测定血清超氧化歧化酶(SOD)、丙二醛(MDA)和肝组织脂质过氧化物(120)水平。结果：湿热证模型大鼠肝细胞出现明显凋亡形态改变,凋亡率明显增加,血清SOD活性下降,MDA含量上升,肝脏IPO含量明显上升。清香散治疗组大鼠肝细胞凋亡率明显下降,血清SOD活性上升,MDA含量下降,肝脏120含量明显下降。结论：清香散具有抑制湿热证大鼠模型氧化过程和抗细胞凋亡的功能。

The Effect of "Qingxiangsan" on Inhibiting Oxidation and Hepatic Cells Apoptosis in the Wet-heat Syndrome Rat Model

Objective:To observe the changes of oxidation and hepatic cells apoptosis in the wet-heat syndrome rat model, and the effect of "qingxiangsan" on inhibiting oxidation and hepatic cells apoptosis. Methods: Wistar rats were divided into wet-heat syndrome group, qingxi-angsan group and normal control group. Flow cytometry and electron microscopy were employed to detect hepatic cells apoptosis. SOD and MDA in the serum, LPO in the hepatic tissue were also estimated. Results: Apoptotic morphogenetic changes, apoptotic rate increasing, activity of SOD decreasing, content of MDA and LPO increasing were obvious in the wet-heat syndrome group.In the "qingxiangsan" group,apoptotic rate deer-casing, activity of SOD increasing, content of MDA and LPO decreasing were examined. Conclusion:"Ringxiangsan" possesses the function of inhibiting oxidation and apoptosis in the wet-heat syndrome rat model.