| 水囊压迫大鼠脑组织致星形胶质细胞中胶质纤维酸性蛋白的变化 |
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| 引用本文： | 亢晓冬,梁国庆,沈礼,孙霞. 水囊压迫大鼠脑组织致星形胶质细胞中胶质纤维酸性蛋白的变化[J]. 杭州医学高等专科学校学报, 2011, 0(3): 166-168,F0002 |
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| 作者姓名： | 亢晓冬 梁国庆 沈礼 孙霞 |
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| 作者单位： | 杭州师范大学实验动物中心;杭州师范大学环境与生命科学学院;杭州师范大学基础医学部; |
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| 基金项目： | 浙江省科技计划项目(2007F80005); 浙江省自然科学基金资助项目(Y2090878); 杭州市科技计划项目(200908333B23) |
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| 摘 要： | 目的研究水囊压迫大鼠颅脑造成损伤后星形胶质细胞的反应。方法将改装后的5F漂浮导管球囊部位全部插入颅内硬膜外后，注射0．5ml无菌生理盐水人球囊使其扩张并维持压力2h。于伤后不同时间将大鼠处死，取脑组织进行HE染色和胶质纤维酸性蛋白（Glialfibrillaryacidicprotein，GFAP）免疫组化染色。结果伤后存活1h组，可见GFAP阳性细胞散在于损伤区周围，但突起较短，反应性星形胶质细胞主要局限于创缘周围。伤后存活24h组，GFAP表达的范围扩大，在创缘及其伤区周围的阳性细胞增多，胞体增大，突起增长，且染色加深。24h在海马组织中已有GFAP阳性细胞表达。伤后存活3d、6d和12d组，阳性细胞的表达继续维持在高水平，且细胞形态各异，胞体变化较大，在创缘出现突起少而粗短的GFAP阳性细胞，而在损伤区周围、海马以及脑干的区域阳性细胞突起粗大不规则，突起进一步增长，胞浆突起染色较深。12d时，GFAP阳性细胞增多达到高峰，阳性细胞着色深，呈深棕黄色。结论水囊压迫造成脑组织损伤后星形胶质细胞的标志性蛋白GFAP表达出现规律性变化，表明水囊压迫的损伤模型可以模拟颅脑血肿的损伤。
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| 关 键 词： | 脑损伤 星形胶质细胞 胶质纤维酸性蛋白 |
Changes of Glial fibrillary Acidic Protein in Astrocytes During Water-sac Pressure-caused Brain Injury |
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| KANG Xiao-dong,LIANG Guo-qing,SHEN Li,SUN Xia. Changes of Glial fibrillary Acidic Protein in Astrocytes During Water-sac Pressure-caused Brain Injury[J]. Journal of Hangzhou Medical College, 2011, 0(3): 166-168,F0002 |
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| Authors: | KANG Xiao-dong LIANG Guo-qing SHEN Li SUN Xia |
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| Affiliation: | KANG Xiao-dong1,LIANG Guo-qing2,SHEN Li3,SUN Xia3(1.Laboratory Animal Center,Hangzhou Normal University,Hangzhou,Zhejiang 310036,China,2.College of Life and Environmental Science,3.Department of Basic Medical Science,China) |
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| Abstract: | Objective To investigate the response of astrocytes to craniocerebral injury caused by water-sac pressure on rat brain. Methods The water sac entirely inserted into encephalic putamen of rat was filled with sterile physiological saline and maintained its size and pressure for 2 hours.Rats were sacrificed at indicated times and their brain tissue were collected to carry out HE staining and immunohistochemical staining. Results In the 1 h injury group,Glial fibrillary acidic protein(GFAP) positive cells with ... |
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| Keywords: | Traumatic brain injury astrocytes glial fibrillary acidic protein |
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