乙肝病毒基因产物和HSP70在肝炎相关性肝癌发生中的作用

目的：探讨乙肝病毒基因产物（HBsAg，HBcAg，HBxAg）和热休克蛋白70（hot shock protein70．HSP70）在乙型肝炎相关性肝癌中的表达及其在肝细胞癌变中的作用。方法：采用组织芯片技术和免疫组织化学染色方法对152例乙型肝炎相关肝细胞肝癌组织、78例乙型肝炎后肝硬化组织中HSP70、HBsAg、HBcAg和HBxAg的表达情况进行检测。结果：与乙型肝炎后肝硬化相比，在乙型肝炎相关性肝癌组织中HBsAg表达显著降低（P=0．000），HBcAg表达显著增高（P=0．000），HBxAg显著增高（P=0．005），HSP70呈现高表达（P=0．000）；相关分析结果显示．HSP70的表达和HBcAg的表达呈显著正相关（P=0．009），而和HBxAg的表达未显示相关性（P=-0．673）。结论：乙肝病毒抗原成分HBcAg、HBxAg以及HSP70参与在乙型肝炎相关性肝癌的发生过程，HBcAg可能通过增加HSP70的表达而参与肝癌的发生。

Effects of HBV Gene Products and HSP70 on Development of Hepatitis-related Hepatocellular Carcinoma

Objective:To study the expression of the products of the HBV genome and HSP70 in hepatitis-related hepatocellular carcinomas and to determine the possible mechanism in the course of carcinogenesis.Methods:Samples from a total of 152 cases with carcinoma and 78 cases with post-hepatitis liver cirrhosis were stained immunohistochemically in tissue array format to detect expression of the HBV antigens HBsAg,HBcAg,HBxAg and HSP70.Results:The expression levels of HBcAg,HBxAg,and HSP70 were significantly higher in carcinoma than in cirrhosis(P=0.000,P=0.005 and P=0.000,respectively) .The expression of HBsAg was significantly lower in carcinoma than in cirrhosis(P=0.000) .Correlation analysis showed that the expression of HSP70 was significantly correlated with the expression of HBcAg(P=0.009) but was not related to the HBxAg(P=0.673) .Conclusion:HBcAg,HBxAg and HSP70 participate in the development of hepatitis-related hepatocellular carcinoma;HBcAg may take part in such a course by a mechanism related to HSP70.HBcAg may be involved in carcinogenesis through an increase in HSP70 expression.