化瘀生脉方对血管吻合术后内皮细胞愈合影响的实验研究

为研究化瘀生脉方对小血管吻合术后内皮细胞愈合机制的影响。采用SD大鼠尾动脉切断后作端端吻合的模型。按用药不同随机分成化瘀生脉组和空白对照组,每组16只。每组再按血管吻合术后22、4、48小时和7天切取标本时间的不同随机分为4组。样本用扫描电镜观察吻合口处内皮细胞的修复过程。结果显示术后2、24、48小时和7天,化瘀生脉组缝线表面完全覆盖率均高于空白对照组,经成组设计两样本比较的秩和检验,统计学上有显著性差异。术后2、24、48小时,化瘀生脉组在针孔及缝线表面的覆盖成分以纤维素、内皮细胞为主,构成比不同于空白对照组,经χ2检验,其差异有非常显著性意义(P<0.01)。表明化瘀生脉方具有抑制血小板在针孔处聚集的作用,能够促进纤维素对针孔及缝线表面的覆盖,能够促进内皮细胞的生长,从而可以防治术后血栓形成。

EFFECT OF STASIS-ELIMINATING AND PULSE-ACTIVATING RECIPE ON ENDOTHELIAL-CELL HEALING AFTER VASCULAR ANASTOMOSIS: An Experimental Study

The objective of the paper is to study the effect of stasis-eliminating and pulse-activating(SEPA) recipe on endothelial-cell healing mechanism after small vessel anastomosis.Thirty-two SD rats were made into models of tail artery end-to-end anastomosis and divided into two groups(n=16 rats): the tested and the blank control,which were transgastrically given SEPA recipe and normal saline,respectively;the samples of each group were taken at 2,24,48 hours and on the 7th day after anastomosis;the endothelial-cell repair processes in the anastomosed sites were observed through electrical microscope scanning.The results showed that at 2,24,48 hours and on the 7th day after anastomosis,the complete covering rate of the sutured surface of the tested was higher than that of the blank control with a significant statistical difference through the rank test;at 2,24 and 48 hours postoperatively,the main covering components in the stys and sutures of the tested were fibrins and endothelial cells and very different from the blank control in the component ratio with a very significant difference(P < 0.01) through the x2 test,suggesting that SEPA recipe functions to inhibit the platelet aggregation around the stys and to promote the fibrin covering sty and sutured surface and the endothelial cells growth and thus to prevent the postoperative thrombosis.