| 脑肿瘤微卫星不稳定性和错配修复基因hMLH1,hMSH2蛋白表？… |
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| 引用本文： | 林瑜,杨树源.脑肿瘤微卫星不稳定性和错配修复基因hMLH1,hMSH2蛋白表？…[J].中华神经外科杂志,2000,16(6):348-352. |
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| 作者姓名： | 林瑜 杨树源 |
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| 作者单位： | 天津医科大学总医院神经外科(林瑜!300052，杨树源!300052，周阅昌!300052)，中国医学科学院血液学研究所实验血液学国家重点实验室(蔡辉国，陈佩贞) |
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| 摘 要： | 目的 检测脑肿瘤微卫星不稳定性（ＭＩＮ）和错配修复（ＭＭＲ）基因蛋白表达的情况。方法 选取１２个微卫星多态性位点，以微卫星序列－ＰＣＲ法和免疫组化技术检测３７例胶质瘤、２４例脑膜瘤及６例神经鞘瘤中ＭＩＮ和ＭＭＲ基因ｈＭＬＨ１、ｈＭＳＨ２蛋白表达。结果 胶质瘤ＭＩＮ发生率为２７％（１０／３７），脑膜瘤ＭＩＮ阳性率仅为４％（１／２４），神经鞘瘤未见微卫生ＤＮＡ异常。ＡＢＣ免疫组化染色发现１０例ｈＭＬＨ
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| 关 键 词： | 脑肿瘤 微卫星不稳定性 MMR基因 hMLH1 hMSH2 |
Analyses of microsatellite instability and altered expression of hMLH1, hMSH2 in human brain tumors |
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| LIN Yu,YANG Shuyuan,ZHOU Yuechang,et al..Analyses of microsatellite instability and altered expression of hMLH1, hMSH2 in human brain tumors[J].Chinese Journal of Neurosurgery,2000,16(6):348-352. |
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| Authors: | LIN Yu YANG Shuyuan ZHOU Yuechang |
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| Institution: | LIN Yu,YANG Shuyuan,ZHOU Yuechang,et al. Department of Neurosurgery,Tianjin Medical University General Hospital,Tianjin 300052 |
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| Abstract: | Objective To investigate the microsatellite instability and altered expression of mismatch repair genes in human brain tumors.Methods Using PCR denatruing PAGE with 12 polymorphic DNA markers to examine MIN, and immunohistochemical analysis to detect the expression of hMLH1, hMSH2 in 67 brain tumor tissues. Results MIN was observed in 10 of 37 gliomas (27%), 1 of 24 meningiomas (4%), but none in schwannomas. Ten of the 67 tumors showed aberrant expression pattern were MIN( ) gliomas.Conclusions Genomic instability should be regarded as a pathogenetic mechanism in human gliomas, and aberrant expression of MMR genes may be associated with human gliomas. Microsatellite instability and alteration of MMR genes was rare in meningiomas and schwannomas. |
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| Keywords: | Human brain tumors Microsatellite instability Mismatch repair genes |
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