Purification of merozoites ofTheileria sergenti from infected bovine erythrocytes |
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| Authors: | C. Sugimoto M. Sato S. Kawazu T. Kamio K. Fujisaki |
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| Affiliation: | (1) National Institute of Animal Health, 305 Tsukuba, Ibaraki, Japan;(2) Present address: Faculty of Veterinary Medicine, Hokkaido University, 060 Sapporo, Japan |
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| Abstract: | A simple and rapid method for purifying merozoites ofTheileria sergenti from infected bovine erythrocytes was developed. Infected erythrocytes were lysed by the use of a cytolytic toxin produced byAeromonas hydrophila and the lysate was subjected to ultracentrifugation in a Percoll discontinuous density gradient. Pure and morphologically intact merozoites free of erythrocyte membrane were recovered from a band formed at the interface of 40% and 60% (by vol.) Percoll solutions. In this merozoite fraction, contamination of erythrocyte membrane proteins was not detected as examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. |
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