依达拉奉对实验性小鼠自身免疫性脑脊髓炎的抑制作用

目的：研究依达拉奉对实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis ， EAE）小鼠的抑制作用。方法将60只雌性C57BL/6小鼠随机分为3组：正常对照组、EAE组、EAE＋依达拉奉组，每组20只。正常对照组用PBS作为对照。后两组应用髓鞘少突胶质细胞糖蛋白35-55、完全福氏佐剂（ CFA）、结核分枝杆菌，制成的抗原乳剂进行造模。免疫2 d后，EAE＋依达拉奉组腹腔注射依达拉奉5 mg/（ kg· d）。3组均监测20 d，每日称重，观察小鼠的摄食和发病情况，并进行神经功能评分。20 d后取小鼠脊髓组织，进行病理学观察。结果 EAE组小鼠的发病率（80％）高于EAE＋依达拉奉组（45％），差异有统计学意义（P＜0．05）。 EAE＋依达拉奉组神经功能评分各个时间点都低于EAE组，差异有统计学意义（P＜0．05），其中，监测10 d后，EAE组神经功能评分（3．6±0．4）级；而EAE＋依达拉奉组评分为（1．3±0．5）级。 HE染色后发现，EAE组小鼠的脊髓内有大量的炎性细胞浸润，白质脱髓鞘改变明显。而EAE＋依达拉奉组小鼠有较少的炎性细胞浸润，且没有白质脱髓鞘改变。结论依达拉奉对EAE的保护作用可能与其清除自由基、减轻炎性反应有关。

Inhibiting effect of edaravone on experimental autoimmune encephalomyelitis in mice

Objective To study the inhibiting effect of edaravone on experimental autoimmune encephalomyelitis （ EAE ） mouse model.Methods Sixty female C57BL /6 mice were randomly divided into three groups： normal control group, EAE group, EAE ＋edaravone group （n=20）, normal control group with PBS as a control .The application of myelin oligodendrocytes glial cells glycoprotein 35-55 and complete Freund ’ s adjuvant （ CFA） and Mycobacterium tuberculosis antigens emulsions were made modeling in the later two groups .After 2 days immunization , EAE＋edaravone group were injected at a dose of 5 mg/（ kg· d） of edaravone .Three groups were monitored 20 days, daily weighing, feeding and incidence were observed , and neurological function was scored .Mouse spinal cord tissue was obtained for pathological examination after 20 days.Results In EAE＋edaravone treated mice , compared with EAE mice, the incidence and neurological function scores were significantly low （P〈0.05）.The incidence in EAE group was 80%, while the incidence in EAE ＋edaravone group mice was 45%.Monitored for 10 days, in EAE group mice, neural function score was 3.6 ±0.4, while in EAE＋edaravone group mice neural function score was 1.3 ±0.5.HE staining found a large number of inflamma-tory cell infiltration in EAE mice spinal cord , and white matter demyelination was obvious .Nevertheless edaravone group mice had fe-wer inflammatory cell infiltration , and no white matter demyelination .Conclusions Protective effect of edaravone on EAE is related to scavenging free radicals and reducing inflammation .