| 环氧化酶-2在肾缺血再灌注损伤的实验研究 |
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| 引用本文： | 农绍军,黄群,梁庆祖. 环氧化酶-2在肾缺血再灌注损伤的实验研究[J]. 右江医学, 2007, 35(3): 248-249 |
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| 作者姓名： | 农绍军 黄群 梁庆祖 |
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| 作者单位： | 右江民族医学院附属医院泌尿外科,广西百色,533000 |
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| 摘 要： | 目的检测大鼠肾缺血再灌注(I/R)损伤90分钟后COX-2的表达,探讨两者的关系。方法构建I/R模型,取30只大鼠平均分为6组,分别于再灌注后0、1.5、3、5、12和24 h处死,采用免疫组化染色分析分为0-4等级,计算COX-2的表达。结果COX-2内皮细胞的表达在再灌注后3~5 h最明显,12~24 h后逐渐减弱。COX-2的表达在再灌注后1.5、3、5、12和24 h均明显高于0 h(P<0.05)。COX-2表达的最高峰后几小时可发现肾(I/R)损伤最明显。结论COX-2表达和肾(I/R)损伤有关。
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| 关 键 词： | 缺血再灌注损伤 肾 环氧化酶 |
| 文章编号： | 1003-1383（2007）03-0248-02 |
| 收稿时间： | 2007-01-10 |
| 修稿时间： | 2007-01-102007-05-21 |
Experimental study of Cyclooxygenase 2 in Renal Ischemia-Reperfusion Injury |
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| NONG Shao-jun,HUANG qun,LIANG Qing-zu. Experimental study of Cyclooxygenase 2 in Renal Ischemia-Reperfusion Injury[J]. Youjiang Medical Journal, 2007, 35(3): 248-249 |
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| Authors: | NONG Shao-jun HUANG qun LIANG Qing-zu |
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| Affiliation: | Department of Urology, The Affiliated Hospital of Youjiang Medical College, Baise Guangxi 533000, China |
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| Abstract: | Objective To explore the expression of cyclooxygenase 2(COX-2) in rats' kidneys after 90 minutes of ischemia-reperfusion(I/R)injury,and study the relation of COX-2 and renal I/R injury.Methods 30 Rats I/R model were randomly divided to 6 groups and sacrificed at 0,1.5,3,5,12,and 24hours after reperfusion.COX-2 expression was detected by immunohistochemical staining,which was graded 0 to 4.Results COX-2 expression was most intense on endothelial cells at 3 and 5 hours after reperfusion and gradually became weaker from 12 to 24 hours after reperfusion.The COX-2 expression index were significantly higher at 1.5,3,5,12,and 24 hours after reperfusion than that at 0 hour(P<0.05).Several hours after the maximum of COX-2 expression the maximum renal I/R injury was observed.Conclusion There was a relationship between COX-2 expression and renal I/R injury. |
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| Keywords: | ischemia reperfusion injury kidney Cyclooxygenase |
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