| 大孔吸附树脂在EC-SOD纯化中的作用 |
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| 引用本文： | 王英燕,王萍,于锋臣,钟传青. 大孔吸附树脂在EC-SOD纯化中的作用[J]. 齐鲁药事, 2008, 27(8) |
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| 作者姓名： | 王英燕 王萍 于锋臣 钟传青 |
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| 作者单位： | 鲁南制药集团股份有限公司,山东,临沂,276006 |
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| 摘 要： | 目的本文主要论述了大孔吸附树脂在EC-SOD的分离与纯化中所起的作用。该研究在大孔吸附树脂的应用方面有很大的开创性意义。方法研究了非极性大孔吸附树脂在蛋白纯化中去除杂蛋白及DNA碎片、色素等成份的效果。结果研究结果表明在33℃,上样量在30%总吸附量,上样速度在20mL.min-1、pH值为7时有很好的吸附解吸附效果,蛋白回收率90%以上。结论非极性大孔吸附树脂对于粗蛋白的纯化去除杂质效果显著,快速方便,成本低,更适于产业化。
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| 关 键 词： | 大孔吸附树脂 包涵体 EC-SOD |
The role of macroporous adsorption resins in purification of EC-SOD |
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| WANG Ying-yan,WANG Ping,YU Feng-chen,ZHONG Chuan-qing. The role of macroporous adsorption resins in purification of EC-SOD[J]. qilu pharmaceutical affairs, 2008, 27(8) |
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| Authors: | WANG Ying-yan WANG Ping YU Feng-chen ZHONG Chuan-qing |
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| Abstract: | OBJECTIVE The role of macroporous adsorption resins in purification of EC-SOD is stated in this article.The research of application of macroporous adsorption resins is momentous.METHODS The non-polarity macroporous adsorption resins can wipe off miscellaneous protein and DNA,pigment,etc.RESULTS The results show that while submitting sample at 33℃,30% of total adsorption quantity,20mL·min-1,pH7,the effect of adsorbing and desorption is best.The recovery rate is above 90%.CONCLUSION Non-polarity macroporous adsorption resins play important role during purification of coarse protein.The research benefits with more purification,which is convenient,low-cost and suitable for industrialization. |
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| Keywords: | EC-SOD |
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