血管内皮生长因子_C和环氧合酶_2表达与口腔鳞癌淋巴结转移的关系

背景与目的： 探讨血管内皮生长因子-C(vascular endothelial growth factor-C，VEGF-C)和环氧合酶-2(cyclooxyenase-2，COX-2)在口腔鳞状细胞癌(oral squamous cell carcinoma，OSCC)中的表达及其与淋巴管生成、淋巴结转移的关系。 材料与方法： 采用免疫组化SP法检测60例OSCC、23例口腔黏膜癌前病变、19例其它口腔黏膜良性病变组织中COX-2、VEGF-C及VEGFR-3受体的表达，计数肿瘤组织中淋巴管密度(lymphatic vessels density，LVD)，结合临床病理因素进行分析。 结果： OSCC中VEGF-C、LVD和COX-2表达明显高于口腔癌前病变和其它良性病变。OSCC中VEGF-C蛋白表达、LVD与淋巴结转移呈明显正相关关系(P<0.05)；COX-2蛋白表达与肿瘤的淋巴结转移、临床分期明显相关(P<0.05)。VEGF-C和COX-2的表达呈正相关(r=0.519,P<0.01)，两者表达与LVD有正相关关系(r=0.661,P<0.01；r=0.485,P<0.01)。 结论： OSCC中VEGF-C和COX-2高表达,COX-2可能参与VEGF-C淋巴管生成通路，在肿瘤淋巴转移中发挥重要作用。

Expression of VEGF-C and COX-2 in Oral Squamous Cell Carcinoma Correlates with Lymph Node Metastasis

BACKGROUND AND AIM:To investigate the expression of VEGF-C,VEGFR-3 and COX-2 in oral squamous cell carcinoma(OSCC),and the correlation with lymphangiogenesis and lymph node metastasis.MATERIALS AND METHODS:Immunohistochemical SP method for VEGF-C,VEGFR-3 and COX-2 were performed in 60 cases of OSCC,23 cases of precancerous change and 19 benign lesion specimens.The lymphatic vessel density(LVD) in the tumor was counted and analyzed with clinicopathologic parameters.RESULTS:Compared with benign and precancerous change,the expressions of VEGF-C,LVD and COX-2 were significantly higher in OSCC(P<0.05).The expressions of VEGF-C and LVD were significantly correlated with lymphatic metastasis(P<0.05);COX-2 was obviously related with clinical stage and lymphatic metastasis(P<0.05).The expression of COX-2 was closely correlated with that of VEGF-C(r=0.519,P<0.01),with LVD positively correlated with their expression levels(r=0.661,P<0.01 and r=0.485,P<0.01,respectively).CONCLUSION:The expression rates of COX-2 and VEGF-C were high in OSCC.COX-2 may participate in VEGF-C lymphangiogenic pathway and play an important role in the lymph node metastasis of OSCC.