趋化因子MCP-1、MIP-1α在EAE小鼠脊髓中的表达

目的:研究单核细胞趋化蛋白(MCP)-1和巨噬细胞炎性蛋白(MIP)-1α与实验性自身免疫性脑脊髓炎(EAE)发病的关系。方法:用髓鞘少突胶质细胞糖蛋白_(35-55)多肽加福氏完全佐剂皮下注射免疫C57BL/6小鼠建立EAE模型,用免疫组织化学方法观察EAE小鼠发病后第0天(初期)、第7天(高峰期)及第30天(恢复期)脊髓中MCP-1、MIP-1α的表达的变化,并通过免疫组化染色标记星形胶质细胞及小胶质细胞,判断MCP-1、MIP-1α的细胞来源。结果: MCP-1、MIP-1α在EAE小鼠发病初期脊髓中有少量表达,发病高峰期表达增高,而恢复期无表达,MCP-1主要由星形胶质细胞产生,MIP-1α主要由小胶质细胞产生;对照组小鼠脊髓中则没有MCP-1、MIP-1α表达。结论:趋化因子MCP-1、MIP-1α在EAE小鼠CNS不同胶质细胞中表达,是EAE发病早期募集免疫反应细胞向CNS浸润的重要致炎性因子。

The expressions of MCP-1 and MIP-1α in spinal cord of EAE mice

Objective:To investigate the role of monocyte chemoattractant protein-1 (MCP-1) and maerophage inflam- matory protein-1 a (MIP-1 a) in the pathogenesis of experimental autoimmune encephalomyelitis (EAE).Methods:EAE models in C57BL/6 mice were induced by injecting subcutaneously myelin oligodendrocyte glycoprotein (35—55) peptide in complete Freund's adjuvant.The expressions of MCP-1 and MIP-1αin the spinal cord at different stages of EAE were observed and the cellular source of MCP-1 or MIP-1αin CNS were identified respectively by staining using cell phenotype markers.Results:Reactive hypertrophic astrocytes were strongly immunoreactive for MCP-1,while MIP-1αwas pro- duced mainly by microglia.The productions in the CNS of these two chemokines were increased to correlate with disease development.Conclusion:Chemokines MCP-1 and MIP-1a are expressed in different glial cells in the CNS of EAE mice and are important proinflammatory cytokines for trafficing immune cells to infiltrate into CNS at initial stage of EAE.