雷公藤多苷纳米乳体外肝肾的细胞毒性

目的探讨雷公藤多苷纳米乳体外对原代培养肝和肾细胞毒性作用。方法培养的大鼠原代肝细胞和肾上皮细胞,分为空白纳米乳,雷公藤多苷片2,8,32,128和512mg·L-1组,雷公藤多苷纳米乳2,8,32,128和512mg·L-1组,作用24h。MTT法测定细胞存活率并计算半数抑制浓度(IC50),自动生化分析仪测定细胞培养液中谷草转氨酶(GOT),谷丙转氨酶(GPT)和乳酸脱氢酶(LDH)活性。结果雷公藤多苷纳米乳2～512mg·L-1可抑制原代培养肝细胞存活,IC50为128.8mg·L-1,是雷公藤多苷片IC50(48.6mg·L-1)的2.65倍;雷公藤多苷纳米乳512mg·L-1对原代肝细胞细胞培养液中GOT,GPT和LDH活性降低作用明显强于雷公藤多苷片组(P<0.05,P<0.01)。雷公藤多苷纳米乳体外对大鼠肾上皮细胞存活亦有明显的抑制作用,IC50为61.7mg·L-1,是雷公藤多苷片IC50(19.5mg·L-1)的3.16倍;雷公藤多苷纳米乳512mg·L-1对原代肾细胞细胞培养液中GOT和LDH活性降低作用明显强于雷公藤多苷片(P<0.05,P<0.01)。结论用纳米乳包合雷公藤多苷,可降低其对肝细胞和肾上皮细胞的毒性作用。

Toxicity of Tripterygium wilfordii glycoside nanoemulsion in hepatic cells and renal epithelial cells

OBJECTIVE To investigate the hepatic cell and renal epithelial cell′s toxicity of Tripterygium wilfordii glycoside nanoemulsion. METHODS The primary cultured hepatic cells and renal epithelial cells of rats were treated with nanoemulsion, T. wilfordii glucoside tablets 2, 8, 32, 128 and 152 mg·L^-1, and T. wilfordii glucoside nanoemulsion 2, 8, 32, 128 and 512 mg·L^-1 for 24 h, respectively. The survival rate of cells was detected by MTT assay. IC₅₀ was calculated and glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT) and lactate dehydrogenase(LDH) activities were determined by using automatic biochemistry analyzer. RESULTS The primary cultured hepatic cell was inhibited after treated with T. wilfordii glycoside nanoemulsion 2-512 mg·L^-1 for 24 h. IC₅₀ of T. wilfordii glycoside nanoemulsion was 128.8 mg·L^-1 for hepatocyte, 2.65 times that of Leigongteng tablets. The content of GPT, GOT and LDH in cell culture medium in T. wilfordii glycoside nanoemulsion group was obviously lower than that of T. wilfordii glucoside tablets groups. The viality of renal epithelial cells treated with T. wilfordii glycoside nanoemulsion was significantly decreased. IC₅₀ of T. wilfordii glycoside nanoemulsion was 61.7 mg·L^-1 for renal epithelial cells, 3.16 times that of T. wilfordii glucoside tablets. The content of GOT and LDH in cell culture medium was lower than that in T. wilfordii glucoside tablets group. CONCLUSION Nanoemulsion could decrease the toxicity of T. wilfordii glycoside in hepatic cells and the renal epithelial cells.