| 丛生形盔珊瑚共附生真菌的分离及其抗菌活性的筛选 |
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| 引用本文： | 徐佳,雷晓凌,佘志刚,陈彬,肖碧红.丛生形盔珊瑚共附生真菌的分离及其抗菌活性的筛选[J].中国海洋药物,2011,30(5):23-28. |
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| 作者姓名： | 徐佳 雷晓凌 佘志刚 陈彬 肖碧红 |
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| 作者单位： | 广东海洋大学食品科技学院;中山大学化学与化学工程学院;西藏高原生物研究所; |
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| 基金项目： | 广东海洋大学基金(E09095); 国家自然科学基金(20972197); 广东省科技计划项目(2010B03030004) |
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| 摘 要： | 目的本试验旨在研究珊瑚共附生真菌抗菌活性。方法采用96孔板培养法结合酶标仪测定。以6种常见的致病或致食品腐败的细菌为指示菌,对其共附生真菌次级代谢产物进行抗菌活性筛选,对抗菌能力及抗菌谱进行研究。结果结果表明:共分离丛生形盔珊瑚共附生真菌19株,均具有不同程度的抗菌活性。高活性菌株占52.63%,其中4-4、4-5两菌株表现出良好的抗菌活性。结论丛生形盔共附生真菌是潜在的抗菌资源,具有较高的应用潜力和综合开发前景。
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| 关 键 词： | 丛生形盔珊瑚 共附生真菌 次级代谢产物 抗菌活性 筛选 |
Isolation of symbiotic fungi from the coral Galaxea fascicularis and screening of their antibacterial activities |
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| XU Jia,LEI Xiao-ling,SHE Zhi-gang,CHEN Bin,XIAO Bi-hong.Isolation of symbiotic fungi from the coral Galaxea fascicularis and screening of their antibacterial activities[J].Chinese Journal of Marine Drugs,2011,30(5):23-28. |
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| Authors: | XU Jia LEI Xiao-ling SHE Zhi-gang CHEN Bin XIAO Bi-hong |
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| Institution: | XU Jia1,LEI Xiao-ling1,SHE Zhi-gang2,CHEN Bin2,3,XIAO Bi-hong1(1.College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524025,China,2.School of Chemistry and Chemical Engineering,Sun Yat-Sen University,Guangzhou 510275,3.Tibetan Plateau Institute of Biology,Lhasa 850001,China) |
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| Abstract: | Objective To study the antibacterial activity of cultured symbiotic fungi isolated from the coral Galaxea fascicularis.Methods The microplate reader was used to detect the antibacterial activities of the secondary metabolites from symbiotic fungi against Bacillus cereus,Bacillus subtilis,Escherichia coli,Staphylococcus epidermidis,Sarcina luteuand,Staphylococcus aureus in sterile 96-well microtitre plates.Results The results showed that all symbiotic fungal strains had antibacterial activity where 52.63% fu... |
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| Keywords: | Galaxea fascicularis symbiotic fungi secondary metabolites antibacterial activity screening |
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