新生儿感染与人类单核细胞表面抗原HLA-DR及CD14的关系

目的　探讨单核细胞表面 CD14、HL A- DR的表达在新生儿感染性疾病发生发展中的作用及与预后的关系。　方法　用双标记流式细胞术对新生儿感染患儿外周血单核细胞 CD14、HL A- DR的表面表达量进行检测 ,检测时间分别为诊断新生儿感染后的第 1、5～ 7天各测 1次 ,同时检测 C-反应蛋白及外周血白细胞。并以 34例非感染新生儿作为对照 ,并分析比较结果。　结果　感染组患儿入院即刻血清 CD14与对照组比较明显降低 (P<0 .0 0 1) ,血清 HL A- DR也显著低于对照组(P<0 .0 0 1) ;感染组患儿经治疗后血清 CD14水平逐渐升高 (P<0 .0 5 ) ,血清 HL A- DR水平较治疗前有所升高 (P<0 .0 1) ;而 2例死亡小儿随着病情的加重 CD14阳性细胞及其细胞中的 HL A- DR发生明显变化 ,呈明显降低趋势。　结论　新生儿感染患儿外周血单核细胞 CD14及 HL A- DR表面表达量均降低 ;且 CD14和 HL A- DR的总体表达水平与患儿病死率呈负相关

Neonatal Infection Correlates with the Expression of CD14、HLA-DR

Objective To explore the possible relationship between the expression of CD14 and HLA-DR on peripheral blood monocytes and progress of the illness in neonatal infection. Methods To measure the expression of CD14 and HLA-DR on peripheral blood monocytes in the 1st, 5th and 7th days after the neonates were diagnosed as infectious diseases by flow cytometry of dual stai-ning techniques. Thirtyfour uninfected neonates were served as controls. Outcomes were analyzed. Results Neonates with infectious diseases had signi-ficantly lower serum CD14(P<0.001)and lower serum HLA-DR(P<0.001).than uninfected neonates It was found that serum CD14 increased significantly after treatment (P<0.05)and serum HLA-DR increased significantly after treatment (P<0.01).The expression of CD14 and HLA-DR on peripheral blood monocytes in 2 cases who died from infection changed significantly. Conclusions The expression of CD14 and HLA-DR on peripheral blood monocytes in neonates with infectious diseases reduce. The level of the expression of CD14 and HA-DR is negatively correlated with the mortality of infected neonates.