女贞子水提液对去卵巢大鼠骨结构和骨代谢的影响研究

目的探讨女贞子水提液对骨质疏松大鼠骨保护作用和骨代谢调节作用。方法用去卵巢方法复制大鼠骨质疏松模型,其中假手术组大鼠仅切除卵巢周围与卵巢体积大小相同的脂肪。实验分为4组:假手术组、模型组、阿伦膦酸钠(0.12 g/kg)组、女贞子水提液(3.5 g/kg)组,每组9只。各组均ig给药,连续12周。实验结束时,用生化法测定血清钙(S-Ca)、血清磷(S-P)、尿钙(U-Ca/Cr)、尿磷(U-P/Cr)及血清高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)和三酰甘油(TG)的量;用ELISA法测血清I型胶原氨基端前肽(PINP)、I型胶原羧基端肽(CTX-I)、骨钙素(OCN)和尿脱氧吡啶林(DPD)的量;用放免法测定血清碱性磷酸酶(ALP)的活性;分别用骨密度仪、Viva CT和万能试验机评价骨组织结构变化。结果女贞子水提液可以明显抑制去卵巢引起的大鼠体质量上升,升高去卵巢大鼠S-Ca、S-P、HDL-C和PINP量,降低U-Ca/Cr和U-P/Cr水平,降低血清中LDL-C、TC、TG、ALP、OCN和CTX-I水平以及尿中DPD的量(P0.05、0.01)。女贞子水提液可以升高去卵巢大鼠的股骨和椎骨骨密度(BMD),改善股骨的微结构,增加骨强度。结论女贞子水提液能升高去卵巢大鼠的BMD,提高骨强度,其作用机制与调节钙、磷代谢,胶原蛋白和非胶原蛋白代谢有关。

Effects of water extract from Ligustri Lucidi Fructus on bone structure and metabolism in ovariectomized rats

Objective To investigate the protecting and regulatory effects of water extract from Fructus Ligustri Lucidi(FLL) on bone structure and bone metabolism in osteoporosis rats. Methods SD female rats were bilaterally ovariectomized to establish osteoporosis model, and Sham operated rats only cut the fat around the ovary. Experimental rats were divided into four groups:Sham operation(SHAM) group, model(OVX) group, alendronic acid sodium(ALN) group, and Fructus Ligustri Lucidi(FLL) group, with nine rats in each group. The rats in FLL group were given FLL water extract(3.5 g/kg) and rats in ALL group were given ALN suspension(0.12 g/kg) by ig administration for 12 weeks. At the end of the experiments, the contents of serum and urine calcium(S-Ca) and phosphorus(S-P), urine creatinine(U-Ca/Cr and U-P/Cr), serum high density lipoprotein(HDL-C) cholesterol, low density lipoprotein(LDL-C) cholesterol, total cholesterol(TC), and triglyceride(TG) were measured by biochemical methods. The levels of collagen I amino terminal peptide(PINP), collagen I carboxyl terminal peptide(CTX-I), osteocalcin(OCN), and urine deoxypyridinoline(DPD) were measured with ELISA. The determination of alkaline phosphatase(ALP) was by radioimmunoassay method. To evaluate the change of bone tissue structure, the bone density instrument, VivaCT, and a universal testing machine were used. Results FLL could inhibit the increased body weight of ovariectomy(OVX) rats, increase S-Ca, S-P, serum HDL and PINP contents, reduce urinary U-Ca/Cr and U-P/Cr ratios, reduce serum LDL-C, TC, TG, ALP, OCN, CTX, and reduce urinary DPD content(P<0.05 or 0.01) in OVX rats. Meanwhile, FLL can elevate the femur head and vertebral bone mineral density, bone micro-structure and bone strength in OVX rats. Conclusion FLL can improve the bone density and bone strength in OVX rats by regulating Ca and P metabolism, collagen and non-collagen metabolism.