| 白英毛状根的培养与薯蓣皂苷元的测定 |
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| 引用本文： | 陈宇,董瑜,张楷燕,孙一铭,程孟琪,张来,孙敏. 白英毛状根的培养与薯蓣皂苷元的测定[J]. 中草药, 2016, 47(7): 1199-1203 |
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| 作者姓名： | 陈宇 董瑜 张楷燕 孙一铭 程孟琪 张来 孙敏 |
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| 作者单位： | 西南大学生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715;西南大学生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715;西南大学生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715;西南大学生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715;安顺学院 贵州省教育厅功能材料与资源化学特色重点实验室, 贵州 安顺 561000;安顺学院 贵州省教育厅功能材料与资源化学特色重点实验室, 贵州 安顺 561000;西南大学生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715 |
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| 基金项目： | 中央高校基本科研业务费专项资金项目(XDJK20120088);贵州省功能材料与资源化学特色重点实验室开放基金项目 |
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| 摘 要： | 目的建立白英Solanum lyratum毛状根诱导与培养体系,筛选出薯蓣皂苷元高产的毛状根无性系。方法利用发根农杆菌C58C1感染白英外植体获得毛状根,建立白英毛状根遗传转化体系。采用HPLC法检测薯蓣皂苷元量。结果菌液浸染时间为10 min、共培养时间为4 d可获得最佳转化效果,毛状根诱导率为83.33%。HPLC检测结果表明,野生型白英植株中叶片的薯蓣皂苷元量最高,为1.742 mg/g。毛状根中薯蓣皂苷元的平均质量分数为4.620 mg/g,是白英叶片的2.652倍。结论通过白英毛状根离体培养,可以高效率地获得薯蓣皂苷元。
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| 关 键 词： | 白英 毛状根 薯蓣皂苷元 HPLC 再生植株 |
| 收稿时间： | 2015-06-26 |
Culture of hairy roots in Solanum lyratum and determination of diosgenin |
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| CHEN Yu,DONG Yu,ZHANG Kai-yan,SUN Yi-ming,CHENG Meng-qi,ZHANG Lai and SUN Min. Culture of hairy roots in Solanum lyratum and determination of diosgenin[J]. Chinese Traditional and Herbal Drugs, 2016, 47(7): 1199-1203 |
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| Authors: | CHEN Yu DONG Yu ZHANG Kai-yan SUN Yi-ming CHENG Meng-qi ZHANG Lai SUN Min |
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| Affiliation: | Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE), SchooL of Life Sciense, Southwest University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE), SchooL of Life Sciense, Southwest University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE), SchooL of Life Sciense, Southwest University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE), SchooL of Life Sciense, Southwest University, Chongqing 400715, China;Special and Key Laboratory of Functional Materials and Resource Chemistry of Guizhou Provincial Education Department, Anshun University, Anshun 561000, China;Special and Key Laboratory of Functional Materials and Resource Chemistry of Guizhou Provincial Education Department, Anshun University, Anshun 561000, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE), SchooL of Life Sciense, Southwest University, Chongqing 400715, China |
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| Abstract: | Objective To establish the introduction and culture system of the hairy roots in Solanum lyratum and to screen the clone of hairy roots with more diosgenin. Methods The explants of S. lyratum were infected by Agrobacterium tumefaciens strain C58C1, to obtain the hairy roots and construct the genetic transformation system of the hairy roots in S. lyratum. HPLC was used to determine the diosgenin in the hairy roots. Results The optimum transformation results were obtained with the max inductivity of hairy roots of 83.33% during infecting time for 10 min by C58C1 and co-cultural time of 4 d. The average content of diosgenin in the hairy roots was 4.620 mg/g, it was 2.652 times as high as that in the leaves(1.742 mg/g) which had the highest diosgenin content in the different tissues of wild type plant of S. lyratum. Conclusion It is an effective way to obtain diosgenin from the hairy roots of S. lyratum. |
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| Keywords: | Solanum lyratum Thunb. hairy roots diosgenin HPLC regeneration plant |
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