| 恶性疟原虫杂合45肽抗原与乙肝病毒表面抗原融合基因在酵母中表达 |
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| 引用本文： | 陈爱社,管惟滨,方振伟,王昌才.恶性疟原虫杂合45肽抗原与乙肝病毒表面抗原融合基因在酵母中表达[J].中国病原生物学杂志,1997(3). |
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| 作者姓名： | 陈爱社 管惟滨 方振伟 王昌才 |
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| 作者单位： | 第一军医大学生物化学教研室，第二军医大学寄生虫学教研室 |
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| 摘 要： | 通过ＰＣＲ扩增ＨＢｓＡｇ基因的Ｓ片段，然后将Ｓ基因与恶性疟原虫杂合４５肽基因连接并克隆到酵母表达质粒ｐＹＥＵｒａ３上。重组质粒被转化到酵母受体菌中。融合基因在酵母中获得表达，表达产物ＨＢｓＡｇ／４５肽，该融合蛋白经亲合层析后被纯化。电镜观察到ＨＢｓＡｇ／４５肽呈颗粒状。Ｄｏｔ┐ＥＬＩＳＡ分析表明恶性疟原虫杂合４５肽抗原可能暴露在颗粒表面
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| 关 键 词： | 恶性疟原虫 融合基因 HBsAg／45肽 酵母表达 |
EXPRESSION OF THE FUSED GENES OF HBsAg AND THE HYBRID 45 MER ANTIGEN OF PLASMODIUM FALCIPARUM IN YEAST |
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| Chen Aishe,Guan Weibin,Fang Zhenwei,Wang Changcai.EXPRESSION OF THE FUSED GENES OF HBsAg AND THE HYBRID 45 MER ANTIGEN OF PLASMODIUM FALCIPARUM IN YEAST[J].Journal of Pathogen Biology,1997(3). |
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| Authors: | Chen Aishe Guan Weibin Fang Zhenwei Wang Changcai |
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| Institution: | Guangzhou 510515 |
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| Abstract: | We amplified S region fragment of HBsAg gene by PCR and connected it with the gene encoding a 45 animoacid peptide of P.falciparum . The complex gene was cloned to yeast expression vector,pYEUra3. The recombinant plasmid was identified and transformed into yeast competent cells. The fused protein HBsAg/45 mer was successfully expressed in yeast and was purified by affinity chromatography. The hybrid HBsAg/45 mer particles were observed by electron microscope. It might be shown by Dot ELISA that the 45 mer would lie on the surface of the particles. |
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| Keywords: | Plasmodium falciparum fused genes HbsAg/45 mer yeast expression |
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