Fixed acid and carbon dioxide bohr effects as functions of hemoglobin-oxygen saturation and erythrocyte pH in the blood of the frog,Rana temporaria

Using a thin film, dynamic recording technique, the pH sensitivity of the oxygen equilibrium (Bohr effect) of whole blood in the frogRana temporaria, and its dependence on CO₂ and fixed acids and on plasma and erythrocyte pH values were measured. Under standard conditions (20°C,P _{CO 2}=14.7 mm Hg, pH=7.65) the oxygen equilibrium could be described by a P₅₀ value of 38 mm Hg andn ₅₀ of 1.8. Hill plots of the oxygen equilibria showed increased cooperativity in oxygen binding with increasing saturation (n ₂₀ 1.2,n ₈₀ 4.0). Values of the fixed acid and CO₂ Bohr factors ({ie7-1} and {ie7-2}, respectively) were similar at specific saturations (S_{20, 50, 80}) but showed saturation dependence with high values occurring at high saturation. The same statements also hold for the intracellular Bohr factors (derived from the relation between blood P₅₀ and erythrocyte pH) although the values of both {ie7-3} and {ie7-4} now were greater than those related to blood pH.