| 高效液相色谱质谱法测定齐墩果酸固体分散物的溶出度研究 |
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| 引用本文： | 莫斌斌,王峰,向大雄,陶昱斐,李焕德. 高效液相色谱质谱法测定齐墩果酸固体分散物的溶出度研究[J]. 中国新药与临床杂志, 2005, 24(4): 290-293 |
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| 作者姓名： | 莫斌斌 王峰 向大雄 陶昱斐 李焕德 |
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| 作者单位： | 1. 中南大学湘雅二医院,药剂科,湖南,长沙,410011
2. 中南大学药学院,湖南,长沙,410078 |
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| 摘 要： | 目的: 建立齐墩果酸高效液相色谱质谱联用(HPLC MS)浓度测定方法,并用于溶出度实验时溶出液中齐墩果酸的测定。方法:采用高效液相色谱质谱联用法测定齐墩果酸固体分散物溶出度实验中齐墩果酸的浓度。结果: 在0. 2 ~12. 8mg·L-1的浓度范围内,齐墩果酸线性关系良好(r= 0. 999 7 ),高、中、低浓度回收率分别为98. 6 %, 99. 3 %, 102. 4 % (n=5),日内RSD低于5. 0 % (n=5),日间RSD低于10. 0 % (n=5)。结论:本方法灵敏度高、分析时间短、杂质干扰少,可用于齐墩果酸溶出度实验的研究及制剂含量的测定。
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| 关 键 词： | 齐墩果酸 色谱法,高压液相 光谱分析,质量 电喷雾电离 溶出度 |
| 文章编号： | 1007-7669(2005)04-0290-04 |
Study of dissolution of oleanolic acid solid dispersion by HPLC-MS |
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| MO Bin-bin,WANG Feng,XIANG Da-xiong,TAO Yu-fei,LI Huan-de. Study of dissolution of oleanolic acid solid dispersion by HPLC-MS[J]. Chinese Journal of New Drugs and Clinical Remedies, 2005, 24(4): 290-293 |
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| Authors: | MO Bin-bin WANG Feng XIANG Da-xiong TAO Yu-fei LI Huan-de |
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| Affiliation: | MO Bin-bin1,WANG Feng 1*,XIANG Da-xiong1,TAO Yu-fei2,LI Huan-de1 |
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| Abstract: | AIM: To develop a sensitive HPLC-MS method for determining of concentration for oleanolic acid (OLA) in solution of solid dispersion dissolution. METHODS: Determination concentration of OLA in dissolution experiment of solid dispersion by HPLC-MS. RESULTS: The fine linear range of the method was 0.2-12.8 mg·L -1 (r= 0.999 7). The limit of detection was 4 μg·L -1 as S/N≥3,the recoveries of the high, medium and low concentrations were 98.6 %, 99.3 %, 102.4 %(n=5),respectively. The intra-day RSD was lower than 5.0 %( n=5) and inter-day RSD was lower than 10.0 %(n=5). CONCLUSION: This method is simple, rapid, sensitive and specific, suitable for applying in the study of OLA. |
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| Keywords: | oleanolic acid chromatography high pressure liguid spectrum analysis mass electrospray ionization dissolution |
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