Effects of epidermal growth factor and/or nerve growth factor on Malassez’s epithelial rest cells in vitro: expression of mRNA for osteopontin,bone morphogenetic protein 2 and vascular endothelial growth factor

Yamawaki K, Matsuzaka K, Kokubu E, Inoue T. Effects of epidermal growth factor and/or nerve growth factor on Malassez’s epithelial rest cells in vitro: expression of mRNA for osteopontin, bone morphogenetic protein 2 and vascular endothelial growth factor. J Periodont Res 2010; 45: 421–427. © 2010 John Wiley & Sons A/S Background and Objective: Malassez’s epithelial rest (MER) cells are involved in the maintenance and homeostasis of the periodontal ligament (PDL). The purpose of this study was to determine the effects of epidermal growth factor (EGF) and/or nerve growth factor (NGF) in vitro on these functions of MER cells. Material and Methods: MER cells from porcine PDL were incubated for 3 or 9 h after the addition of EGF and/or NGF to final concentrations of 10 ng/mL. Cells cultured without those growth factors were used as controls. The expression of mRNA for osteopontin, bone morphogenetic protein 2 (BMP‐2) and vascular endothelial growth factor (VEGF) was analyzed using quantitative RT‐PCR. Results: There was a decrease in the expression of osteopontin mRNA by MER cells treated for 9 h with NGF and the level of mRNA expressed was lower than that of the control and EGF‐treated groups. The expression of BMP‐2 mRNA by MER cells treated with NGF for 9 h also decreased, and was lower than that of the control and EGF‐treated groups. The expression of VEGF mRNA by MER cells treated with EGF for 3 or 9 h was higher than in the control and NGF‐treated groups. The expression of VEGF mRNA was lower in MER cells treated with NGF for 3 and 9 h than in the control and EGF‐treated groups, and decreased from 3 to 9 h of treatment. EGF stimulated MER cells to secrete VEGF, which suggests that EGF plays an important role in maintaining the homeostasis of the PDL. NGF acts on MER cells to inhibit calcification in the PDL. Furthermore, in the EGF+NGF‐treated MER cells, expression of mRNA for BMP‐2 and VEGF was similar to that of the NGF‐treated group, but cell proliferation and expression of osteopontin mRNA were similar to that of the EGF‐treated group. Conclusion: EGF and NGF play important roles in maintaining the PDL.