17%乙二胺四乙酸冲洗液对牙本质粪肠球菌黏附的影响

目的 研究17%乙二胺四乙酸（EDTA）冲洗液对促进牙本质粪肠球菌黏附的影响。方法 将48例半劈样本及12例牙本质片样本随机分为3个实验组和1个对照组,实验组分别经17%EDTA处理1、3、5 min,对照组经生理盐水处理5 min,接种粪肠球菌后通过扫描电子显微镜、激光共聚焦电子显微镜（CLSM）、菌落形成单位及组织学方法进行细菌黏附量的评价。结果 除组织学革兰染色显示,1 min组与对照组、3 min组的细菌侵入深度无明显差异（P>0.05）外,其余结果显示,实验组细菌生物膜厚度、侵入牙本质小管深度（CLSM测量）及菌落计数均大于对照组,差异具有统计学意义（P<0.05）;3个实验组之间比较,差异也具有统计学意义（P< 0.05）。结论 17%EDTA冲洗液可促进粪肠球菌对牙本质的黏附,且黏附量随处理时间的延长而增加。

Effect of 17% ethylenediaminetetraacetic acid irrigation on the dentin adhesion of Enterococcus faecalis

Objective This work aims to uncover the promoting effect of 17% ethylenediaminetetraacetic acid (EDTA) irrigation on the dentin adhesion of Enterococcus faecalis (E. faecalis). Methods Forty-eight half split samples and twelve dentin slices were randomly divided into three experimental groups and one control group. The experimental groups and the control group were soaked with EDTA in different time lengths and with normal saline, respectively. E. faecalis was inoculated, and its dentin adhesion was measured via scanning electron microscopy, confocal laser scanning microscopy (CLSM), colony forming unit counts, and histological Gram staining. Results According to histological Gram staining, the depth showed no statistically significant differences between 1 min group and the control group, 1 min group and 3 min groups (P>0.05). E. faecalis intruded in the dentine tubules (measured by CLSM), and the thickness of the biofilm on the dentin surface and the colony numbers of experimental groups were greater than those of the control group (P<0.05). The differences between the three experimental groups were statistically significant (P<0.05). Conclusion EDTA (17%) irrigation can promote E. faecalis adhesion to dentin. This adhesion would in turn prolong EDTA treatment time.